그란자임B

그란자임B C화학적 특성, 용도, 생산

일반 설명

Granzyme B, a 247 amino acid polypeptide, contains a leader sequence, which is cleaved by a signal peptidase and a two amino acid prodomain, which is cleaved by the lysosomal cysteine protease DPPI. The recombinant granzyme B is expressed in Pichia pastoris as the mature form and appears on SDS-PAGE as a triplet (~34, 32, and 30 kDa) due to three different glycosylations.

Biochem/physiol Actions

Cytotoxic granules secreted by natural killer (NK) cells and cytotoxic T Lymphocytes cells are part of the mechanism used for protecting the organism from virus infected and tumor cells. Granzyme B, a serine protease, is the most prominent granzyme in a family of 11 found in the cytotoxic granules. The granzymes enter the target cell with the assistance of perforin, a critical molecule of the cytotoxic granules. In the target cell, the granzymes act on specific substrates involved with the cell death via apoptosis.Granzyme B is a neutral serine protease that cleaves aspartic acid residues, inducing cell death by various pathways. It can cleave and activate most of the caspases in vitro and in vivo resulting in a massive amplification of the caspase dependent apoptotic pathway. In addition, granzyme B cleaves directly downstream caspase substrates as PARP DNA-PK2 and DFF45/ICAD leading to cell death. This pathway bypasses inhibition of apoptosis by viral caspase inhibitors found in virus infected cells. It has been shown that granzyme B is capable of inducing cytochrome C release from the mitochondria in a caspase independent way.

그란자임B 준비 용품 및 원자재

원자재

준비 용품

그란자임B 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Creative Enzymes	1-516-855-7709	info@creative-enzymes.com	United States	8748	58
3B Pharmachem (Wuhan) International Co.,Ltd.	821-50328103-801 18930552037	3bsc@sina.com	China	15839	69
Wuhan Chemstan Biotechnology Co., Ltd.	027-65317797 15926423062	422450190@qq.com	China	10308	58
Shanghai Huzhen Industrial Co., LTD	021-60345367 13916550749	sales@shzbio.com	China	9965	58
Biolab Reagents	18108604356	info@biolabreagent.com	China	9868	58
CUSABIO TECHNOLOGY LLC	027-87196173	cusabio@163.com	China	6066	58
Merck KGaA	21-20338288	ordercn@merckgroup.com	China	6394	58
Beijing Biosynthesis Biotechnology Co Ltd	400-9019800	sales@bioss.com.cn	China	6102	58

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