HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
화학적 성질
White crystalline powder
용도
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
정의
ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
일반 설명
HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
생물학적 활성
HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
Biotechnological Applications
HEPES serves as a buffering agent and transfection reagent that mediates cytoplasmic delivery of proteins into cells, and mixing it with proteins such as antibodies, recombinant proteins, and small peptides yields protein–HEPES mixtures suitable for transfection into cultured cells[1]. As a zwitterionic buffering agent in cell culture media, HEPES activates MiT/TFE-dependent lysosomal-autophagic gene networks and induces lysosome biogenesis, while its addition to culture media also triggers a lysosomal stress-related inflammatory phenotype and affects host-immune gene programs in RAW macrophages[2]. HEPES serves as a building block for molecular engineering of biomedical polymers, functioning as a pendant group in the zwitterionic polymer polyHEPES-pz intended for biomedical applications[3].
Purification Methods
Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]
