| Company Name: |
Alabiotech Inc. |
| Tel: |
001-619-354-5251 |
| Email: |
sales@alabiotech-e.com |
|
| | Phosphoramidon Basic information |
| Product Name: | Phosphoramidon | | Synonyms: | N-(ALPHA-RHAMNOPYRANOSYLOXYHYDROXYPHOSPHINYL)-L-LEUCYL-L-TRYPTOPHAN DISODIUM SALT DIHYDRATE;N-(ALPHA-RHAMNOPYRANOSYLOXYHYDROXYPHOSPHINYL)-L-LEUCYL-L-TRYPTOPHAN (SODIUM SALT);N-(ALPHA-RHAMNOPYRANOSYLPHOSPHONO)-L-LEUCYL-L-TRYPTOPHAN DISODIUM SALT;N-[N-[[(6-deoxy-alpha-L-mannopyranosyl)oxy]hydroxyphosphinyl]-L-leucyl]-L-tryptophan;N-[N-[(6-Deoxy-α-L-mannopyranosyloxy)hydroxyphosphinyl]-L-Leu-]-L-Trp-OH;L-Tryptophan,N-[[(6-deoxy-a-L-mannopyranosyl)oxy]hydroxyphosphinyl]-L-leucyl-;eucyl)-;l-tryptophan,n-(n-(((6-deoxy-alpha-l-mannopyranosyl)oxy)hydroxyphosphinyl)-l-l | | CAS: | 36357-77-4 | | MF: | C23H34N3O10P | | MW: | 543.51 | | EINECS: | 252-996-3 | | Product Categories: | peptides;Pepetides | | Mol File: | 36357-77-4.mol |  |
| | Phosphoramidon Chemical Properties |
| density | 1.48±0.1 g/cm3(Predicted) | | storage temp. | −20°C | | solubility | H2O: 1 mM Stock solution stable for 1 month at –20° C. | | pka | -0.66±0.50(Predicted) |
| | Phosphoramidon Usage And Synthesis |
| Uses | Phosphoramidon, a microbial metabolite, is a specific metalloprotease thermolysin inhibitor with an IC50 of 0.4 μg/mL. Phosphoramidon also inhibits endothelin-converting enzyme (ECE), neutral endopeptidase (NEP), and angiotensin-converting enzyme (ACE) with IC50 values of 3.5, 0.034, and 78 μM, respectively[1][2][3]. | | Definition | ChEBI: A dipeptide isolated from the cultures of Streptomyces tanashiensis. | | Enzyme inhibitor | This glycosyl phosphoramidopeptide (FWfree-acid = 543.51 g/mol; CAS 36357-77-4), also systematically named as N-(a-rhamnopyranosyloxyhydroxyphosphinyl)- L-leucyl-L-tryptophan, is a slow-binding thermolysin inhibitor (Ki = 30 nM). Note: Phosphoramidon is an epimer of talopeptin. Stock 1 mg/mL aqueous solutions of phosphoramidon are stable for one month at –20°C. Target(s): atrolysin A; atrolysin C; bacillolysin; carboxypeptidase C, weakly inhibited; coccolysin; cytosol alanyl aminopeptidase, weakly inhibited; dactylysin; endothelin-converting enzyme; endothelin-converting enzyme 2; fungalysin; gametolysin; granzyme B; Legionella metalloendopeptidase; leucyl aminopeptidase, weakly inhibited; Listeria metalloprotease Mpl; lysine carboxypeptidase, or lysine(arginine) carboxypeptidase; carboxypeptidase N; mycolysin; neprilysin, or enkephalinase, Ki = 0.8 nM; oligopeptidase O; peptidyl-dipeptidase A, or angiotensin I-converting enzyme; pitrilysin, or protease Pi; pseudolysin, or Pseudomonas aeruginosa elastase; pyruvate decarboxylase; thermolysin (27,28,41,43- 53); urethanase; vibriolysin; and Xaa-Pro aminopeptidase, or aminopeptidase P. | | in vivo | Phosphoramidon (0.25 mg/kg per min; i.v.) suppresses the hypertensive effect of big endothelin-1 in rats[4].
Phosphoramidon (1-30 mg/kg; i.v.; once) blocks the pressor activity of porcine big endothelin-1-(1-39) in rats[5]. | Animal Model: | Male Sprague-Dawley rats[4] | | Dosage: | 0.25 mg/kg per min | | Administration: | Intravenous injection | | Result: | Markedly suppressed the hypertensive effect of big endothelin-1. |
| | IC 50 | Microbial Metabolite | | References | [1] Umezawa S, , et al. A new microbial metabolite phosphoramidon (isolation and structure). Tetrahedron Letters, 1972, 13(1): 97-100.
[2] Suda H, et al. A thermolysin inhibitor produced by actinomycetes: phosphoramidon. The Journal of antibiotics, 1973, 26(10): 621-623.
[3] Kukkola PJ, et al. Differential structure-activity relationships of phosphoramidon analogues for inhibition of three metalloproteases: endothelin-converting enzyme, neutral endopeptidase, and angiotensin-converting enzyme.J CardiovascPharmacol. 1995;26Suppl 3:S65-8. PMID:8587470
[4] Matsumura Y, et al. Phosphoramidon, a metalloproteinase inhibitor, suppresses the hypertensive effect of big endothelin-1. Eur J Pharmacol. 1990 Aug 21;185(1):103-6. DOI:10.1016/0014-2999(90)90216-s
[5] McMahon EG, et al. Phosphoramidon blocks the pressor activity of porcine big endothelin-1-(1-39) in vivo and conversion of big endothelin-1-(1-39) to endothelin-1-(1-21) in vitro. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):703-7. DOI:10.1073/pnas.88.3.703 |
| | Phosphoramidon Preparation Products And Raw materials |
|