盐酸丁双胍
| 中文名称 | 盐酸丁双胍 |
|---|---|
| 中文同义词 | 盐酸丁双胍;盐酸丁二胍/盐酸丁福明;盐酸丁二胍;盐酸丁福明;丁福明盐酸盐;正丁基亚胺基二碳亚胺二酰胺盐酸盐;盐酸丁双胍 标准品;丁双胍盐酸盐 |
| 英文名称 | BuforMin Hydrochloride |
| 英文同义词 | 1-Butylbigyanidine hydrochloride;1-Butylbiguanide Hydrochloride;N-ButylidodicarboniMidic DiaMide Hydrochloride;PanforMin;Sindiatil;TideMol;Ziavetine;Adebit |
| CAS号 | 1190-53-0 |
| 分子式 | C6H16ClN5 |
| 分子量 | 193.68 |
| EINECS号 | 214-723-6 |
| 相关类别 | 对照品;Amines;Intermediates & Fine Chemicals;Pharmaceuticals |
| Mol文件 | 1190-53-0.mol |
| 结构式 |  |
盐酸丁双胍 性质
| 熔点 | 177-179C |
|---|---|
| 储存条件 | Inert atmosphere,Store in freezer, under -20°C |
| 溶解度 | DMSO（少许）、甲醇（少许）、水（少许） |
| 形态 | 固体 |
| 颜色 | 白色至类白色 |
| 水溶解性 | H2O: 25mg/mL, clear |
| 稳定性 | 吸湿性 |
| InChI | 1S/C6H15N5.ClH/c1-2-3-4-10-6(9)11-5(7)8;/h2-4H2,1H3,(H6,7,8,9,10,11);1H |
| InChIKey | KKLWSPPIRBIEOV-UHFFFAOYSA-N |
| SMILES | [Cl-].N\C(=N/CCCC)\N=C(N)N.[H+] |
| CAS 数据库 | 1190-53-0 |
Buformin hydrochloride (0-10 mM; 5 days) inhibits SKBR3 and BT474 cells growth as a concentration-dependent manner, exhibits IC 50 values of 246.7 μM and 98.6 μM for erbB-2-overexpressing SKBR3 and BT474 cells, respectively.Buformin hydrochloride (0-3 mM; 48 hours) increases the percentage of cells in G0/G1 phase and reduced the percentage of cells in S phase, especially in the SKBR3 cells.Buformin hydrochloride (0-3 mM; 24 hours) suppresses RTK activation, including erbB-2 and IGF1R signaling downstream, and Akt activation/phosphorylation is inhibited in both SKBR3 and BT474 cells.
Cell Viability Assay
| Cell Line: | ErbB-2-overexpressing SKBR3 and BT474 cells |
| Concentration: | 0 μM, 1 μM, 3 μM, 10 μM, 30 μM, 100 μM, 300 μM, 1, 3, or 10 mM |
| Incubation Time: | 5 days |
| Result: | Reduced cell viability in erbB-2-overexpressing breast cells. |
Cell Cycle Analysis
| Cell Line: | ErbB-2-overexpressing SKBR3 and BT474 cells |
| Concentration: | 0.5 mM; 1 mM; 3 mM |
| Incubation Time: | 48 hours |
| Result: | Increased cells arresting in G0/G1 phase. |
Western Blot Analysis
| Cell Line: | ErbB-2-overexpressing SKBR3 and BT474 cells |
| Concentration: | 0 mM, 0.1 mM, 0.3 mM, 1 mM, or 3 mM |
| Incubation Time: | 24 hours |
| Result: | Decreased p-AMPK, p-p706S, p-ERK1/2 expression in a concentration-dependent manner. |
Buformin hydrochloride (oral administation; 7.6 mmol/kg of chow; 7 days) exhibits significantly reduced tumor volumes and weights, and hinders mammary morphogenesis and proliferation in MMTV-erbB-2 transgenic mice.
| Animal Model: | Female MMTV-erbB-2 transgenic mice |
| Dosage: | 7.6 mmol/kg |
| Administration: | Oral administation; 7 days |
| Result: | Inhibited mammary syngeneic tumor growth in MMTV-erbB-2 transgenic mice. |