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ACHROMOPEPTIDASE

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ACHROMOPEPTIDASE Basic information
Product Name:ACHROMOPEPTIDASE
Synonyms:achromopeptidase crude;achromopeptidase partially purified;achromopeptidase from achromobacter lyticus;Achromobacter protease I;Achromobacter proteinase I;Endo-Lys-C protease;Endopeptidase Lys-C;Endoprotease LysC
CAS:123175-82-6
MF:
MW:0
EINECS:
Product Categories:
Mol File:Mol File
ACHROMOPEPTIDASE Structure
ACHROMOPEPTIDASE Chemical Properties
storage temp. −20°C
form partially purified powder
color Colorless to light yellow
Safety Information
Hazard Codes Xn
Risk Statements 42/43-42
Safety Statements 36-45-22
WGK Germany 3
MSDS Information
ProviderLanguage
SigmaAldrich English
ACHROMOPEPTIDASE Usage And Synthesis
DescriptionEndoproteinase Lys-C, Sequencing Grade, can be used for specific cleavage of peptides.Endoproteinase Lys-C, Sequencing Grade, is a widely used serine protease, that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of lysin. Endoproteinase Lys-C is isolated from Lysobacter enzymogenes and is supplied as a lyophilizate.
UsesLysyl endopeptidase, Achromobacter sp (Lys-C) catalyzes carboxyl oxygen exchange reaction. Lysyl endopeptidase has higher substrate binding affinities and higher catalytic rates at the acidic pHs than at the alkaline pHs[1].
ApplicationUse Endoproteinase Lys-C, Sequencing Grade, for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.
References[1] Hajkova D, et al. pH dependency of the carboxyl oxygen exchange reaction catalyzed by lysyl endopeptidase and trypsin. J Proteome Res. 2006 Jul;5(7):1667-73. DOI:10.1021/pr060033z
ACHROMOPEPTIDASE Preparation Products And Raw materials
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