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| | 4-Methylumbelliferyl beta-D-galactoside Basic information |
| | 4-Methylumbelliferyl beta-D-galactoside Chemical Properties |
| Melting point | 258-263 °C (dec.) | | alpha | -64 º (c=1, pyridine, after) | | Boiling point | 626.9±55.0 °C(Predicted) | | density | 1.522±0.06 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | Soluble in DMSO (40 mg/ml) | | pka | 12.73±0.70(Predicted) | | form | Powder | | color | White to slightly off-white | | Water Solubility | soluble | | Sensitive | Moisture Sensitive & Hygroscopic | | BRN | 94673 | | Stability: | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month. | | InChI | InChI=1/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16-/s3 | | InChIKey | YUDPTGPSBJVHCN-ZDHZLMEDNA-N | | SMILES | O(C1=CC2OC(C=C(C)C=2C=C1)=O)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O |&1:13,15,18,20,22,r| | | CAS DataBase Reference | 6160-78-7(CAS DataBase Reference) |
| Hazard Codes | T+ | | Risk Statements | 26/27/28 | | Safety Statements | 24/25 | | WGK Germany | 3 | | F | 3-10-21 | | HS Code | 29329900 | | Storage Class | 11 - Combustible Solids |
| | 4-Methylumbelliferyl beta-D-galactoside Usage And Synthesis |
| Description | 4-MUG (6160-78-7) is a versatile fluorogenic substrate for -galactosidase1 for use in cell extracts or purified enzyme preparations.1-3 This substrate produces a water soluble blue fluorescent coumarin fluorophore. Ex: 342 nm; Em: 441 nm | | Chemical Properties | White Powder | | Uses | It is a substrate used for fluorometric assay for β-galactosidase. It is used to detect E-coli n the fluorogenic medium. | | Uses | Substrate for fluorometric assay of -D-galactosidase | | Definition | ChEBI: A beta-D-galactoside having a 4-methylumbelliferyl substituent at the anomeric position. | | References | [1] NORMAN H. L. CHIU Amanda L W. Measuring β-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter[J]. Current protocols in toxicology, 2018, 74 1. DOI:10.1002/cptx.35
[2] KASSIANI KYTIDOU. Nicotiana benthamiana α-galactosidase A1.1 can functionally complement human α-galactosidase A deficiency associated with Fabry disease.[J]. The Journal of Biological Chemistry, 2018: 10042-10058. DOI:10.1074/jbc.ra118.001774
[3] ALICIA HERNÁNDEZ-GUZMÁN. Purification and characterization of an extracellular β-glucosidase from Sporothrix schenckii[J]. FEBS Open Bio, 2016, 6 11: 1067-1077. DOI:10.1002/2211-5463.12108 |
| | 4-Methylumbelliferyl beta-D-galactoside Preparation Products And Raw materials |
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