2-硝基苯-beta-D-半乳糖苷
| 中文名称 | 2-硝基苯-beta-D-半乳糖苷 |
|---|---|
| 中文同义词 | 2-硝基苯基-~-D-半乳糖苷, 98+%;邻硝基苯-Α-D-吡喃葡萄糖苷/2-硝基苯-Α-D-吡喃葡萄糖苷/ONPG;2-硝基苯基-Β-D-吡喃半乳糖苷(动物源);邻硝基苯-BETA-D-半乳糖苷;2-硝基苯基-Β-D-吡喃半乳糖苷(植物源);2-硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Β-D-半乳糖苷(ONPG) |
| 英文名称 | 2-Nitrophenyl-beta-D-galactopyranoside |
| 英文同义词 | 2-Nitrophenyl hexopyranoside;2-Nitrophenyl-beta-delta-galactopyranoside;2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol;o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem;beta-D-Galactopyranoside, 2-nitrophenyl;beta-d-galactopyranoside,2-nitrophenyl;Galactopyranoside, o-nitrophenyl, beta-D-;o-Nitrophenyl beta-galactoside |
| CAS号 | 369-07-3 |
| 分子式 | C12H15NO8 |
| 分子量 | 301.25 |
| EINECS号 | 206-716-1 |
| 相关类别 | 化学试剂;分子生物试剂-多肽与蛋白;碳水化合物;专业试剂;中间体;糖类（碳水化合物）;糖类;检测标志试剂;糖类化合物;氨基酸;核苷;糖苷系列;多肽与蛋白;生化试剂-酶类;即用型溶液;Substrates;Biochemistry;Galactose;Enzyme substrates;Carbohydrates & Derivatives;生化试剂;酶底物;酶类;酶活性检测;酶及活性检测;试剂;优势产品;其他原料;精细化工原料;化学原料;通用生化试剂-酶与辅酶;还原剂;化工中间体;其它;化学试剂;Glycosides;Sugars;substrate |
| Mol文件 | 369-07-3.mol |
| 结构式 |  |
2-硝基苯-beta-D-半乳糖苷 性质
| 熔点 | 186-193 °C (dec.) |
|---|---|
| 比旋光度 | -70 º (c=1, H2O) |
| 沸点 | 442.39°C (rough estimate) |
| 密度 | 1.3709 (rough estimate) |
| 折射率 | -68.8 ° (C=1, H2O) |
| 储存条件 | 2-8°C |
| 溶解度 | DMSO（微溶）、水（微溶、加热、超声处理） |
| 酸度系数(pKa) | 12.69±0.70(Predicted) |
| 形态 | 粉末 |
| 颜色 | 白色至微黄色 |
| 旋光度 (Optical Rotation) | [α]20/D 70±1°, c = 1% in H2O |
| 水溶解性 | Soluble in water. |
| BRN | 92207 |
| InChI | 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 |
| InChIKey | KUWPCJHYPSUOFW-RMPHRYRLSA-N |
| SMILES | OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O |
| CAS 数据库 | 369-07-3(CAS DataBase Reference) |
| NIST化学物质信息 | Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3) |
The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (K m ), turnover numbers (k cat ), and catalytic efficiencies (k cat /K m ) of Gal308 for ONPG and lactose are determined. The k cat and K m values are 464.7±7.8 s -1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s -1 and 7.1±0.8 mM for lactose, respectively. The k cat /K m value of the enzyme for ONPG (172.1 s -1 mM -1 ) is 4.6-fold higher than that for lactose (37.2 s -1 mM -1 ), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose.
安全信息
| 危险品标志 | Xi |
|---|---|
| 危险类别码 | 36/37/38 |
| 安全说明 | 22-24/25-36-26 |
| WGK Germany | 3 |
| F | 3-10-21 |
| 海关编码 | 29400090 |
| 存储类别 | 11 - 可燃固体 |
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