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| Product Name: | QX77 | | Synonyms: | QX77;CMA activator;CMA ACTIVATOR;QX77;QX-77;QX 77;Acetamide, N-[4-(7-chloro-2H-1,4-benzoxazin-3-yl)phenyl]-;N-(4-(7-Chloro-2H-benzo[b][1,4]oxazin-3-yl)phenyl)acetamide;LAMP2A,QX 77,Inhibitor,Rab11,QX77,inhibit,QX-77,CMA,pluripotency,MEFs,Autophagy | | CAS: | 1798331-92-6 | | MF: | C16H13ClN2O2 | | MW: | 300.74 | | EINECS: | | | Product Categories: | | | Mol File: | 1798331-92-6.mol |  |
| Boiling point | 514.5±50.0 °C(Predicted) | | density | 1.32±0.1 g/cm3(Predicted) | | storage temp. | Store at -20°C | | solubility | DMF: 25mg/mL; DMF:PBS (pH 7.2) (1:2): 0.3mg/mL; DMSO: 15mg/mL | | form | A solid | | pka | 14+-.0.70(Predicted) | | color | Light yellow to yellow | | InChI | 1S/C16H13ClN2O2/c1-10(20)18-13-5-2-11(3-6-13)15-9-21-16-8-12(17)4-7-14(16)19-15/h2-9,19H,1H3,(H,18,20) | | InChIKey | FVNBPTNXXWCRJJ-UHFFFAOYSA-N | | SMILES | Clc1cc2c(cc1)NC(=CO2)c3ccc(cc3)NC(=O)C |
| WGK Germany | WGK 3 | | Storage Class | 13 - Non Combustible Solids |
| Uses | QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells[1]. QX77 can impede self-renewal and promote differentiation of ES cells[2]. | | Biological Activity | QX77 is a chaperone-mediated autophagy (CMA) activator derived from the atypical retinoid AR7 th at activates CMA by antagonizing retinoic acid receptor-α (RARα) signaling. QX77 rescues defective trafficking & lysosomal localization of the CMA receptor LAMP2A in cystinotic Ctns-/- MEFs and CTNS-KO human proximal tubule cells (PTCs) by restoring Rab11 expression and Rab11-positive vesicles trafficking to the level seen in wild-type cells (20 μM, 48 hrs). CMA activation by QX77 treatment (20 μM, 5 days) also significantly reduces toxic α-synuclein (α-syn) accumulation in PD patients iPSCs-derived astrocytes with LRRK2 G2019S mutation. | | References | [1] Jinzhong Zhang, et al. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. J Biol Chem. 2017 Jun 23;292(25):10328-10346 DOI:10.1074/jbc.M116.764076
[2] Yi Xu, et al. Chaperone-mediated autophagy regulates the pluripotency of embryonic stem cells. Science. 2020 Jul 24;369(6502):397-403. DOI:10.1126/science.abb4467 |
| | QX77 Preparation Products And Raw materials |
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