DAF-FM DA manufacturers
- DAF-FM DA
-
-
2026-07-22
- CAS:254109-22-3
- Purity:
- Supply Ability: 10g
- DAF-FM DA
-
-
2026-06-30
- CAS:254109-22-3
- Min. Order: 1KG
- Purity: 98%
- Supply Ability: 1000kg
|
| | DAF-FM DA Basic information |
| Product Name: | DAF-FM DA | | Synonyms: | 4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy);4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one;DAF-FM DA solution;DAF-FM DA (cell permeable);-[9H]xanthen]-3-one;Diaminofluorescein-FM diacetate;-difluoro-spiro[isobenzofuran-1(3H);DAF-FM DA | | CAS: | 254109-22-3 | | MF: | C25H18F2N2O7 | | MW: | 496.42 | | EINECS: | | | Product Categories: | | | Mol File: | 254109-22-3.mol |  |
| | DAF-FM DA Chemical Properties |
| Boiling point | 695.7±55.0 °C(Predicted) | | density | 1.56±0.1 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | Soluble in dimethyl sulfoxide | | pka | 4.20 ± 0.20, most basic, temperature:
25 °C | | form | Pale yellow solution | | color | clear clear, colorless | | Appearance | beige solid | | λmax | 365 nm (MeOH) | | Biological Applications | Detecting bacteria/microorganisms; nitric oxide indicator, | | InChI | 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3 | | InChIKey | BEVHTVRRVVEMEF-UHFFFAOYSA-N | | SMILES | CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35 |
| WGK Germany | 3 | | Storage Class | 11 - Combustible Solids |
| | DAF-FM DA Usage And Synthesis |
| Description | DAF-FM diacetate (DAF-FM DA) is a cell-permeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM DA passively diffuses across cellular membranes and, once inside cells, is deacetylated by intracellular esterases and converted to cell-impermeant form — DAF-FM.
The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .
The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.
DAF-FM DA should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously. | | Uses | DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495 | | Uses | DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs). | | Biochem/physiol Actions | DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases. | | in vivo | Using sections of zebrafish embryos live-stained with DAF-FM DA (5 μM), could confirm that the fluorescent signals were predominantly located in areas of ongoing bone formation[1]. | | storage | Store at -20°C | | Excitation / Emission Maximum | 495 nm/515 nm |
| | DAF-FM DA Preparation Products And Raw materials |
|