泽兰黄酮
| 中文名称 | 泽兰黄酮 |
|---|---|
| 中文同义词 | 泽兰黄酮;6-甲氧基藤黄菌素;泽兰叶黄素;6-甲氧基木犀草素, 来源于泽兰;泽兰黄酮(6-甲氧基藤黄菌素;2-(3,4-二羟基苯基)-5,7-二羟基-6-甲氧基-4H-苯并吡喃-4-酮;泽兰黄酮/6-甲氧基藤黄菌素/泽兰叶黄素;6-甲氧基藤黄菌素,10 MM DMSO 溶液 |
| 英文名称 | 6-METHOXYLUTEOLIN |
| 英文同义词 | EUPAFOLIN;NSC 122416;Eupafolin(Nepetin,NSC 122416);Eurafolin;Eupafloin 6-Methoxyluteolin;6-METHOXYLUTEOLIN;NEPETIN;2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-6-methoxy-4H-1-benzopyran-4-one |
| CAS号 | 520-11-6 |
| 分子式 | C16H12O7 |
| 分子量 | 316.26 |
| EINECS号 | |
| 相关类别 | 标准品;对照品;中药对照品;植物提取物;黄酮;Tetra-substituted Flavones;标准品 -中药标准品;标准品，对照品;化工原料 |
| Mol文件 | 520-11-6.mol |
| 结构式 |  |
泽兰黄酮 性质
| 熔点 | 267°C |
|---|---|
| 沸点 | 659.1±55.0 °C(Predicted) |
| 密度 | 1.605 |
| 闪点 | 90℃ |
| 储存条件 | 2-8°C |
| 溶解度 | 溶于甲醇； |
| 酸度系数(pKa) | 6.48±0.40(Predicted) |
| 形态 | 粉末 |
| 颜色 | 粘黄色 |
| 水溶解性 | slightly soluble in water |
| InChI | InChI=1S/C16H12O7/c1-22-16-11(20)6-13-14(15(16)21)10(19)5-12(23-13)7-2-3-8(17)9(18)4-7/h2-6,17-18,20-21H,1H3 |
| InChIKey | FHHSEFRSDKWJKJ-UHFFFAOYSA-N |
| SMILES | C1(C2=CC=C(O)C(O)=C2)OC2=CC(O)=C(OC)C(O)=C2C(=O)C=1 |
泽兰黄酮(6-Methoxyluteolin) 是从 Eupatorium ballotaefolium HBK 分离的天然类黄酮，具有有效的抗炎活性。Nepetin 在 ARPE-19 细胞中抑制 IL-6，IL-8 和 MCP-1 分泌，IC50 值分别为 4.43 μM、3.42 μM 和 4.17 μM。
Pretreatment with Nepetin dose-dependently inhibited IL-6, IL-8 and MCP-1 secretion with
IC
50
values of 4.43, 3.42 and 4.17 μM, respectively.
Nepetin (2.5-10 μM; 25 hours; ARPE-19 cells) treatment suppresses IL-1β-induced cytokine (IL-6, IL-8 and MCP-1) expression at mRNA level in ARPE-19 cells.
Nepetin (2.5-10 μM; 1.5 hours; ARPE-19 cells) treatment dose-dependently inhibits phosphorylation of IKKα/β and IκBα, and nuclear translocation of p65. Nepetin decreases the level of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells.
RT-PCR
| Cell Line: | ARPE-19 cells |
| Concentration: | 2.5 μM, 5 μM, 10 μM |
| Incubation Time: | 25 hours |
| Result: | Suppressed the mRNA expression of IL-6, IL-8 and MCP-1 in ARPE-19 cells. |
Western Blot Analysis
| Cell Line: | ARPE-19 cells |
| Concentration: | 2.5 μM, 5 μM, 10 μM |
| Incubation Time: | 1.5 hours |
| Result: | Dose-dependently inhibited phosphorylation of IKKα/β and IκBα, and nuclear translocation of p65. Decreased the level of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells. |