FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 manufacturers
- Fluo-4 AM
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- $0.00 / 1mg
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2025-07-14
- CAS:273221-67-3
- Min. Order:
- Purity:
- Supply Ability: 10g
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| | FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Basic information |
| Product Name: | FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 | | Synonyms: | FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5;Fluo 4-AM;4-(6-acetoxymethoxy-2,7-difluoro-3-oxo-9-xanthenyl)-4'-methyl-2,2'-(ethylenedioxy)dianiline-n,n,n',n'-tetraacetic acid tetrakis(acetoxymethyl) ester;N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester;Fluo4amester;Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*;Fluo-4-(acetoxymethyl) ester;Glycine, N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, (acetyloxy)methyl ester | | CAS: | 273221-67-3 | | MF: | C51H50F2N2O23 | | MW: | 1096.94 | | EINECS: | | | Product Categories: | Xanthene | | Mol File: | 273221-67-3.mol |  |
| | FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Chemical Properties |
| Melting point | >200℃ | | Boiling point | 1063.2±65.0 °C(Predicted) | | density | 1.47 | | storage temp. | −20°C | | solubility | DMSO: soluble | | pka | 1.92±0.50(Predicted) | | form | Solid | | color | Brown to reddish brown | | Appearance | orange-red powder | | λmax | 456nm | | Biological Applications | Calcium indicator | | InChIKey | QOMNQGZXFYNBNG-UHFFFAOYSA-N | | SMILES | C(OCOC(C)=O)(=O)CN(C1=CC=C(C2C3=C(C=C(OCOC(C)=O)C(F)=C3)OC3C=2C=C(F)C(=O)C=3)C=C1OCCOC1=CC(C)=CC=C1N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O |
| | FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Usage And Synthesis |
| Description | Fluo-4 AM is a cell-permeable Ca2+-indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon Ca2+-binding (excitation/emission λ at 494/506 nm). Fluo-4 AM is used for visualization and measurement of intracellular Ca2+. It is well suited for fluorometric and imaging applications such as microscopy, flow cytometry, spectrofluorometry, and fluorometric high-throughput microplate screening assays [1].
Fluo-4 AM is similar in structure and spectral properties to the widely used Ca2+-indicator, Fluo-3, but it has certain advantages over Fluo-3, such as brighter fluorescence emission, high rate of cell permeation, and a Kd for Ca2+ in buffer of 345 nM. Because of its higher fluorescence emission intensity, Fluo-4 AM can be used at lower intracellular concentrations, making its use less toxic for live cells.
As Fluo-4 AM does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. In vivo cell imaging with Fluo-4 AM is usually performed within one or two hours after loading, but the dye can be re-loaded to cells if it is needed. Fluo-4 AM can also be fixed in situ by EDC/EDAC for downstream immunofluorescence studies.
Fluo-4 AM has low solubility in the water. It is recommended to prepare 1 mM stock solution in labeling grade DMSO prior to cell loading. Use the final concentration of 1-5 μM and incubation at 37 °C for 15-60 min as a start point of your protocol. | | Uses | Fluorescent Ca2+ indicator dye; cell-permeable AM ester, insensitive to magnesium. | | Uses | Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator. Displays high fluorescence emission. Excitation/emission λ 494/516 nm. | | storage | Store at -20°C | | Abs/Em Maxima | 494/518 nm | | Excitation / Emission Maximum | 494 nm/506 nm |
| | FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Preparation Products And Raw materials |
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