CKI-7 DIHYDROCHLORIDE
| 中文名称 | CKI-7 DIHYDROCHLORIDE |
|---|---|
| 中文同义词 | N-(2-氨基乙基)-5-氯异喹啉-8-磺酰胺二盐酸盐;CKI-7 二盐酸盐;CKI-7 盐酸盐;N-(2-氨基乙基)-5-氯异喹啉-8-磺酰胺二盐酸盐,10 MM DMSO 溶液;CKI-7 HYDROCHLORIDE |CAS 1177141-67-1;CKI-7 dihydrochloride[C0742] |
| 英文名称 | N-(2-Aminoethyl)-5-chloroisoquinoline-8-sulfonamide Dihydrochloride |
| 英文同义词 | N-(2-Aminoethyl)-5-chloroisoquinoline-8-sulfonamide Dihydrochloride;CKI-7 dihydrochlorid;N-(2-Aminoethyl)-5-chloro-8-isoquinolinesulfonamide dihydrochloride;CKI-7 2HCL;Serum and glucocorticoid-regulated kinase,inhibit,nestin,S6K,Inhibitor,CK1,Casein Kinase,Ribosomal S6 Kinase (RSK),differentiation,SGK,CKI-7,Cyclin dependent kinase,neural,Serum-glucocorticoid regulated kinase,S6K1,MSK1,CDK,CKI 7,Wnt,CKI7,βIII-tubulin,Sox1;CKI-7 (hydrochloride);CKI-7, 10 mM in DMSO;CKI-7 dihydrochloride[C0742] |
| CAS号 | 1177141-67-1 |
| 分子式 | C11H14Cl3N3O2S |
| 分子量 | 358.67176 |
| EINECS号 | |
| 相关类别 | 合成;Inhibitors;Protein Kinase Inhibitors and Activators;All Inhibitors |
| Mol文件 | 1177141-67-1.mol |
| 结构式 |  |
CKI-7 DIHYDROCHLORIDE 性质
| 熔点 | 187-189°C |
|---|---|
| 储存条件 | -20°C |
| 溶解度 | 在水中的溶解度>5mg/mL |
| 形态 | 固体 |
| 颜色 | 白色至浅棕褐色 |
| 水溶解性 | H2O: >5mg/mL |
| InChI | 1S/C11H12ClN3O2S.2ClH/c12-10-1-2-11(18(16,17)15-6-4-13)9-7-14-5-3-8(9)10;;/h1-3,5,7,15H,4,6,13H2;2*1H |
| InChIKey | JUAVTXYOCISSSL-UHFFFAOYSA-N |
| SMILES | Cl[H].Cl[H].NCCNS(=O)(=O)c1ccc(Cl)c2ccncc12 |
|
CK1 6 μM (IC 50 ) |
CK1 8.5 μM (Ki) |
Cdc7
|
SGK
|
S6K1
|
MSK1
|
CKI-7 (0.1-10 μM; 5 days; ES cells) treatment significantly increases the expression of the early neuroectodermal marker Sox1 and the number of cells positive for the neural markers nestin and βIII-tubulin, in a concentration-dependent manner.
CKI-7 (5 μM; 5 days; ES cells) treatment suppresses SFEB-induced β-catenin stabilization on day 5, indicating that CKI-7 inhibits Wnt signaling.
RT-PCR
| Cell Line: | Mouse ES cells |
| Concentration: | 0.1-10 μM |
| Incubation Time: | 5 days |
| Result: | Significantly increased the expression of the early neuroectodermal marker Sox1 and the number of cells positive for the neural markers nestin and βIII-tubulin, in a concentration-dependent manner. |
Western Blot Analysis
| Cell Line: | Mouse ES cells |
| Concentration: | 5 μM |
| Incubation Time: | 5 days |
| Result: | Suppressed SFEB-induced β-catenin stabilization on day 5. |
In vivo dose-dependent anti-tumor activity of CKI-7 is demonstrated in a SCID-Beige mouse systemic tumor model utilzing a recently isolated Philadelphia chromosome positive acute lymphoblastic leukemia cell line. Standard cell cycle synchronization studies established that exposure to CKI-7 results in cell cycle dependent caspase 3 activation and apoptotic cell death.