- 5-Aminofluorescein
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- $0.00 / 1kg
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2026-03-06
- CAS:3326-34-9
- Min. Order: 1kg
- Purity: 98%
- Supply Ability: Customise
- 5-Aminofluorescein
-
- $1.00 / 1KG
-
2026-03-06
- CAS:3326-34-9
- Min. Order: 1KG
- Purity: 99%
- Supply Ability: 10 mt
- 5-Aminofluorescein
-
- $99.00/ kg
-
2026-03-02
- CAS:3326-34-9
- Min. Order: 0.001kg
- Purity: 99%
- Supply Ability: 5000
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| | 5-Aminofluorescein Basic information |
| Product Name: | 5-Aminofluorescein | | Synonyms: | 5-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one
Fluoresceinamine;5-amino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one;FLUORESCEINAMINE;FLUORESCEINAMINE ISOMER I;FLUORESCEINAMINE, ISOMER 1;5-amino-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid;5-AMINO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE;5-AMINOFLUORESCEIN ISOMER I | | CAS: | 3326-34-9 | | MF: | C20H13NO5 | | MW: | 347.32 | | EINECS: | 222-043-6 | | Product Categories: | marker;Fluorescent Labels and Indicators;Fluorescent Labels & Indicators | | Mol File: | 3326-34-9.mol |  |
| | 5-Aminofluorescein Chemical Properties |
| Melting point | 223 °C (dec.)(lit.) | | Boiling point | 481.89°C (rough estimate) | | density | 1.3724 (rough estimate) | | refractive index | 1.5180 (estimate) | | storage temp. | room temp | | solubility | DMF: 5 mg/ml; DMSO: 5 mg/ml | | pka | 9.34±0.20(Predicted) | | form | powder | | color | red-brown | | Appearance | Solid Powder | | Water Solubility | Soluble in water (partly), methanol (10 mg/ml), and acetone. | | λmax | 496 nm | | BRN | 48395 | | Stability: | Light and Moisture Sensitive | | Major Application | diagnostic assay manufacturing
hematology
histology | | InChI | 1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2 | | InChIKey | GZAJOEGTZDUSKS-UHFFFAOYSA-N | | SMILES | Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35 | | CAS DataBase Reference | 3326-34-9(CAS DataBase Reference) | | EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-amino-3',6'-dihydroxy- (3326-34-9) |
| Hazard Codes | Xn | | Risk Statements | 22-36/37/38 | | Safety Statements | 26 | | WGK Germany | 3 | | F | 10 | | TSCA | TSCA listed | | HS Code | 32049090 | | Storage Class | 11 - Combustible Solids | | Hazard Classifications | Acute Tox. 4 Oral
Eye Irrit. 2
Skin Irrit. 2
STOT SE 3 |
| | 5-Aminofluorescein Usage And Synthesis |
| Description | 5-AMF is a fluorescent probe for carboxylic acids and glutamine, and it also can be coupled to aldehydes and ketones. | | Chemical Properties | Dark Red Solid | | Uses | Glycosaminoglycuronans react with 5-aminofluorescein to yield fluorescent derivatives. 5-aminofluorescein is also used to prepare FITC Isomer I (F-011, F-020). | | Uses | 5-aminofluorescein is also used to prepare FITC Isomer I (CDX-F0011, CDX-F0020). It is a chemical used as molecular probe and important as fluorescent dye and for derivatives. Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens. | | Definition | ChEBI: 5-aminofluorescein is a primary amino compound that is fluorescein carrying an amino substituent at C-5. Building block/intermediate for the synthesis of the fluorescent dye flourescein; also used to produce N-(fluorescein-5-yl)maleamic acid. It derives from a fluorescein (lactone form). | | Preparation |
The preparation of 5-aminofluorescein is carried out either by first saponification of 5-nitrofluorescein acetates with alcohol alkali, followed by reduction of the obtained 5-nitrofluorescein in a solution of alcohol alkali with hydrazine hydrate on a nickel catalyst to obtain 5-aminofluorescein , Or by boiling in an aqueous solution of sodium sulfide. The restoration of the nitro group and the saponification of acetates in the second case occurs in one stage.
| | General Description | Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins. |
| | 5-Aminofluorescein Preparation Products And Raw materials |
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