Rp-8-pCPT-Cyclic GMPS (sodium salt)

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Rp-8-pCPT-Cyclic GMPS (sodium salt) Basic information
Product Name:Rp-8-pCPT-Cyclic GMPS (sodium salt)
Synonyms:Rp-8-pCPT-Cyclic GMPS (sodium salt);8-[(4-Chlorophenyl)thio]-guanosine-cyclic 3',5'-[hydrogen [P(R)]-phosphorothioate] sodium;Rp-8-pCPT-cGMPS sodium;Rp-8-pCPT-Cyclic GMPS sodium salt (Rp-8-pCPT-cGMPS);3-({6-amino-9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-9H-purin-8-yl}amino)propanoic acid
CAS:208445-07-2
MF:C16H16ClN5NaO6PS2
MW:527.87
EINECS:
Product Categories:
Mol File:208445-07-2.mol
Rp-8-pCPT-Cyclic GMPS (sodium salt) Structure
Rp-8-pCPT-Cyclic GMPS (sodium salt) Chemical Properties
storage temp. Store at -20°C
solubility Soluble in H2O
form crystalline solid
Safety Information
MSDS Information
Rp-8-pCPT-Cyclic GMPS (sodium salt) Usage And Synthesis
UsesRp-8-pCPT-Cyclic GMPS Sodium Salt acts as a stable cell-permeable analog which competetively inhibits cGMP-dependant protein kinases affecting the nervous system.
Biological Activityrp-8-pcpt-cyclic gmps is a gmp-dependent protein kinases (cgks) inhibitor.appreciation of cgmp as a distinct intracellular second messenger is reported to be closely followed by an intensive search for effector proteins in various organisms. a cgmp-dependent protein kinase (cgk) has been found in arthropods resulting in the eventual isolation of cgk from mammalian tissues.
in vitroprevious study found that rp-8-pcpt-cyclic gmps could selectively inhibit cgk activity in intact human platelets. the ic50 value of rp-8-pcpt-cyclic gmps for cgk ii was 114-fold lower than that for cgk iα in the presence of 1 mm cgmp. in the presence of 10 mm cgmp, the ic50 values of rp-8-pcpt-cyclic gmps for cgk iα and cgk ii increased 3- and 11-fold, respectively. in addition, millimolar concentrations of rp-8-pcpt-cyclic gmps could fully activate both enzymes, a phenomenon that was observed previously for cgk ii. because substitutions at the 8-position of the guanine ring are poorly tolerated by cgk ib, these results suggested that the rp-isomer of 8-pcpt-cgmps might be a selective activator or inhibitor, respectively, of cgk ii compared to the cgk i isoforms [1].
in vivo

Rp-8-pCPT-cGMPS sodium (5 nM, intrathecally, once a day for 3 days) inhibits the cGMP-cGKI pathway, suppresses tumor cell implantation (TCI)-induced thermal hyperalgesia and mechanical allodynia in Sprague-Dawley rats model[1].

Animal Model:Bone cancer pain induced by tumor cell implantation (TCI) in Sprague-Dawley rats[1].
Dosage:0.5 mmol/L, 20 μL
Administration:intrathecally, once a day for 3 days
Result:Delayed and suppressed TCI-induced thermal hyperalgesia and mechanical allodynia.
IC 5018.3 and 0.16 μm for cgk iα and cgk ii, respectively.
storageStore at -20°C
references[1] gamm, d. m.,francis, s.h.,angelotti, t.p., et al. the type ii isoform of cgmp-dependent protein kinase is dimeric and possesses regulatory and catalytic properties distinct from the type i isoforms. the journal of biological chemisty 270(45), 27380-27388 (1995).
Rp-8-pCPT-Cyclic GMPS (sodium salt) Preparation Products And Raw materials
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