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| | Proadifen hydrochloride Basic information |
| Product Name: | Proadifen hydrochloride | | Synonyms: | SKF-525A HYDROCHLORIDE >98% (PROADIFEN);ALPHA-PHENYL-ALPHA-PROPYLBENZENEACETIC ACID 2-[DIETHYLAMINO]ETHYL ESTER, HCL;ALPHA-PHENYL-ALPHA-PROPYLBENZENEACETIC ACID 2-[DIETHYLAMINO]ETHYL ESTER HYDROCHLORIDE;SK AND F 525A HYDROCHLORIDE;SKF-525A HCL;SKF-525A, HYDROCHLORIDE;diethylaminoethanolesterofdiphenylpropylaceticacidhydrochloride;nsc39690 | | CAS: | 62-68-0 | | MF: | C23H32ClNO2 | | MW: | 389.96 | | EINECS: | 688-917-8 | | Product Categories: | Inhibitors;All Inhibitors;Amines;Aromatics;CLORINE | | Mol File: | 62-68-0.mol |  |
| | Proadifen hydrochloride Chemical Properties |
| Melting point | 122-123°C | | storage temp. | Inert atmosphere,Store in freezer, under -20°C | | solubility | Soluble in DMSO (up to 20 mg/ml) | | form | White solid | | color | Lustrous crystals from Me2CO/pet ether | | Water Solubility | Soluble in methanol or water | | Stability: | Store in Freezer | | Major Application | forensics and toxicology
pharmaceutical (small molecule) | | InChI | 1S/C23H31NO2.ClH/c1-4-17-23(20-13-9-7-10-14-20,21-15-11-8-12-16-21)22(25)26-19-18-24(5-2)6-3;/h7-16H,4-6,17-19H2,1-3H3;1H | | InChIKey | FHIKZROVIDCMJA-UHFFFAOYSA-N | | SMILES | Cl.CCCC(C(=O)OCCN(CC)CC)(c1ccccc1)c2ccccc2 | | EPA Substance Registry System | Proadifen hydrochloride (62-68-0) |
| Hazard Codes | Xn | | Risk Statements | 22 | | Safety Statements | 36-24/25 | | WGK Germany | 3 | | RTECS | YV7175000 | | HS Code | 29221990 | | Storage Class | 11 - Combustible Solids | | Toxicity | LD50 oral in rat: 2140mg/kg |
| | Proadifen hydrochloride Usage And Synthesis |
| Description | SKF 525A is a widely used, nonspecific cytochrome P (CYP)450 inhibitor that demonstrates 100% inhibition of the various CYP450 isoforms at 1-100 μM. It therefore potentiates the effects of many different drugs by inhibiting their metabolism (IC50 values in the μM range when tested using human liver microsomes). SKF 525A inhibits CYP450-dependent arachidonic acid conversion to active EET metabolites, antagonizing the recovery of functional calcium pools. It also acts as a noncompetitive inhibitor of acetylcholine nicotinic receptors (IC50 = 19 μM in mouse skeletal muscle). | | Chemical Properties | Off-White Crystalline Solid | | Uses | anti-infective | | Uses | An inhibitor of NOS1, AChR, cytochrome P-450, and KIR6.1. | | Uses | Cytochrome P-459 inhibitor; blocks glibenclamide-sensitive K+ channels; inhibits neuronal nitric oxide synthetase; stimulates endothelial cell prostacyclin while inhibiting platelet thromboxane synthesis | | Uses | Proadifen hydrochloride is an inhibitor of NOS1, AChR, cytochrome P-450, and KIR6. Proadifen hydrochloride bind to the protein moiety of P-450, also reduced the hypoxic response. of Proadifen hydrochloride have good antiarrhythmic effects. SKF-525A substantially reduced the depletion of cardiac norepinephrine. | | Safety Profile | Poison by intraperitoneal, subcutaneous, and intravenous routes. Moderately toxic by ingestion. Experimental reproductive effects. When heated to decomposition it emits very toxic fumes of NOx and HCl. | | in vitro | previous study found that when incubated with human liver microsomes, skf525a could undergo cyp-dependent oxidative n-deethylation to its secondary amine metabolite skf8742. in addition, skf525a and its metabolite and primary amine analog all inhibited cyp2b6-, cyp2c9-, cyp2c19-, cyp2d6-, as well as cyp3a-selective reactions to various degrees but had little effect on cyp1a2, cyp2a6, and cyp2e1 reactions [1]. | | in vivo | animal study found that skf 525a at 1.5-9 mg/kg could reduce or abolish the hypertensive effect of mcn-a-343, dmpp and nicotine, but could neither noticeably affect the hypertensive effect of tyramine, adrenaline and noradrenaline, nor the hypotensive effect of acetylcholine and orciprenaline. thus, skf 525a was able to block the rat sympathetic ganglia and the adrenal medulla and such blockade was non-specific. moreover, the blockade might result from the stabilizing effect of skf 525a on postsynaptic membranes of the sympathetic ganglia and the adrenal medulla [2]. | | IC 50 | in the μm range when tested using human liver microsomes | | References | [1] MICHAEL R FRANKLIN L B H. 2-Diethylaminoethyl-2,2-diphenylvalerate-HCl (SKF525A) revisited: comparative cytochrome P450 inhibition in human liver microsomes by SKF525A, its metabolites, and SKF-acid and SKF-alcohol.[J]. Drug Metabolism and Disposition, 2008, 36 12: 2539-2546. DOI:10.1124/dmd.108.023549
[2] G SUAREZ-KURTZ C P B. Sites of action of SKF 525-A in nerve and muscle.[J]. Journal of Pharmacology and Experimental Therapeutics, 1970, 172 1: 33-43.
[3] GUILLERMO SPITZMAUL. The local anaesthetics proadifen and adiphenine inhibit nicotinic receptors by different molecular mechanisms[J]. British Journal of Pharmacology, 2009, 157 5: 804-817. DOI:10.1111/j.1476-5381.2009.00214.x
[4] R J PRINCE S M S. Acetylcholine and epibatidine binding to muscle acetylcholine receptors distinguish between concerted and uncoupled models.[J]. The Journal of Biological Chemistry, 1999, 274 28: 19623-19629. DOI:10.1074/jbc.274.28.19623
[5] HIDENARI SAKUTA Ikuo Y. Inhibition by SKF 525A and quinacrine of endogenous glibenclamide-sensitive K+ channels in follicle-enclosed Xenopus oocytes[J]. European journal of pharmacology, 1994, 252 1: Pages 117-121. DOI:10.1016/0014-2999(94)90583-5 |
| | Proadifen hydrochloride Preparation Products And Raw materials |
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