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| | Po-Pro 3 Basic information |
| Product Name: | Po-Pro 3 | | Synonyms: | BENZOXAZOLIUM, 3-METHYL-2-[3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-PYRIDINYLIDENE]-1-PROPENYL]-, DIIODIDE;3-METHYL-2-((E)-3-[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-1,3-BENZOXAZOL-3-IUM DIIODIDE;Po-pro 3 Iodide;Po-Pro 3 | | CAS: | 161016-55-3 | | MF: | C22H29I2N3O | | MW: | 605.29 | | EINECS: | | | Product Categories: | Cyanine | | Mol File: | 161016-55-3.mol |  |
| | Po-Pro 3 Chemical Properties |
| Melting point | >250℃ | | solubility | Soluble in dimethyl sulfoxide | | form | powder | | color | Yellow-brown | | Appearance | red solution | | λmax | 539 nm | | ex/em | 539/567 nm | | ε(extinction coefficient) | 87900 cm−1M−1 | | Φ(quantum yield) | 0.57 | | Biological Applications | Nucleic acid hybridization; detecting nucleic acids,cells; nucleic acid fingerprinting; nucleic acid fragment sizing; nucleic acid amplification |
| | Po-Pro 3 Usage And Synthesis |
| Description | PO-TAP-3 (Oxazole Orange Monomer, also known as PO-PRO®-3) is a yellow fluorescent carbocyanine monomeric dye. PO-TAP-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.
The bright fluorescence signal and low background make PO-TAP-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.
The dye is used to image cell nuclei and cytoplasm, label bacteria and sperms, count reticulocytes, perform nucleic acid amplification and hybridization, and as a temperature sensor. | | Chemical Properties | ex/em: 539/567 nm
Extinction coefficient (ε): 87900 cm1M1
Quantum yield (Φ): 0.57 | | Definition | ChEBI: Po-Pro-3 is a cyanine dye and an organic iodide salt. It has a role as a fluorochrome. It contains a Po-Pro-3(2+). | | Abs/Em Maxima | 531 (complex)/568 (complex) nm |
| | Po-Pro 3 Preparation Products And Raw materials |
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