Bz-Nle-Lys-Arg-Arg-AMC manufacturers
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| | Bz-Nle-Lys-Arg-Arg-AMC Basic information |
| Product Name: | Bz-Nle-Lys-Arg-Arg-AMC | | Synonyms: | Bz-Nle-KRR-AMC;Bz-Nle-Lys-Arg-Arg-AMC trifluoroacetate salt;Bz-nLKRR-AMC;BZ-NLKRR-AMC;BZ-NLE-LYS-ARG-ARG-AMC;863975-32-0;Bz-Nle-KRR-AMC (hydrochloride);L-Argininamide, N-benzoyl-L-norleucyl-L-lysyl-L-arginyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-;Benzoyl-Norleucyl-Lysyl-Arginyl-Arginyl-7-Amino-4-Methylcoumarin;Bz-Nle-Lys-Arg-Arg-AMC | | CAS: | 863975-32-0 | | MF: | C41H60N12O7 | | MW: | 832.99 | | EINECS: | | | Product Categories: | | | Mol File: | 863975-32-0.mol |  |
| | Bz-Nle-Lys-Arg-Arg-AMC Chemical Properties |
| density | 1.366±0.14 g/cm3(Temp: 25 °C; Press: 760 Torr)(predicted) | | storage temp. | Store at -20°C | | solubility | DMF: 30 mg/ml; DMSO: 30 mg/ml; DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml; Ethanol: 20 mg/ml | | form | A solid | | pka | 12.464±0.20(predicted) | | Sequence | {Bz-Nle}-Lys-Arg-Arg-AMC |
| | Bz-Nle-Lys-Arg-Arg-AMC Usage And Synthesis |
| Description | Bz-Nle-KRR-AMC is a fluorogenic peptide substrate for yellow fever virus (YFV) non-structural 3 (NS3) and dengue virus (DV) NS2B/3 serine proteases. Upon enzymatic cleavage by YFV NS3 or DV NS2B/3 serine proteases, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify YFV NS3 or DV NS2B/3 serine protease activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. | | Uses | Bz-Nle-Lys-Arg-Arg-AMC can be used for protease activity assay. In 0.1mL Tris-HCl buffer (50 mM, pH 7.8), Bz-Nle-Lys-Arg-Arg-AMC was hydrolyzed, and the release rate of AMC was observed and calculated at λex/λem=380 nm/460 nm[1]. | | References | [1] Phoo WW, et al. Crystal structures of full length DENV4 NS2B-NS3 reveal the dynamic interaction between NS2B and NS3. Antiviral Res. 2020 Oct;182:104900. DOI:10.1016/j.antiviral.2020.104900 |
| | Bz-Nle-Lys-Arg-Arg-AMC Preparation Products And Raw materials |
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