Suberoylanilide hydroxamic acid (SAHA) is a histone deacetylase (HDAC) inhibitor. Coumarin-Suberoylanilide hydroxamic acid (c-SAHA) is a SAHA derivative where the anilino "cap" group is replaced by 7-amino-4-methylcoumarin to produce a fluorescent probe that competitively binds HDAC. coumarin-SAHA is used to determine binding affinities and dissociation off-rates of HDAC enzyme-inhibitor complexes.
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