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| Product Name: | Methyl Green zinc chloride salt | | Synonyms: | METHYL GREEN, ZINC CHLORIDE;METHYL GREEN, ZINC CHLORIDE SALT;LIGHT GREEN B;DOUBLE GREEN SF;CI NO 42590;CI 42590;ETHYL GREEN ZINC CHLORIDE SALT;Methyl green, crystal violet free, ( | | CAS: | 7114-03-6 | | MF: | C27H35BrCl3N3Zn | | MW: | 653.23 | | EINECS: | 230-415-4 | | Product Categories: | Triphenylmethane | | Mol File: | 7114-03-6.mol |  |
| | Methyl Green zinc chloride salt Chemical Properties |
| Melting point | >300 °C(lit.) | | bulk density | 300kg/m3 | | storage temp. | Store at RT. | | solubility | Soluble in water, ethanol; insoluble in xylene | | Colour Index | 42590 | | form | Solid | | color | Green | | PH Range | Yellow (0.1) to greenish-blue (2.3) | | PH | 4.6 (20°C, 10g/L in H2O) | | Water Solubility | 70 g/L (20 ºC) | | λmax | 629 nm, 423 nm | | ε(extinction coefficient) | ≥14000 at 311-317 in water at 0.005g/L
≥50000 at 629-635 in water at 0.005g/L
≥9000 at 420-426 in water at 0.005g/L | | Stability: | Stable. Incompatible with strong oxidizing agents. | | Biological Applications | Detecting phosphates,polyphosphates,tocopherol,tocopherol acetate | | Major Application | diagnostic assay manufacturing
hematology
histology | | InChI | 1S/C27H35N3.4ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4 | | InChIKey | ZKOGXCSOLMZRAZ-UHFFFAOYSA-J |
| Hazard Codes | Xi,N,C | | Risk Statements | 36/37/38-50/53-34-22 | | Safety Statements | 26-36-61-60-45-36/37/39 | | WGK Germany | 3 | | HS Code | 29215900 | | Storage Class | 8A - Combustible, corrosive hazardous materials | | Hazard Classifications | Aquatic Chronic 2
Carc. 2
Eye Dam. 1
Skin Corr. 1B
STOT SE 3 |
| | Methyl Green zinc chloride salt Usage And Synthesis |
| Description | Methyl Green, Zinc chloride salt (chemical formula: C27H35Cl4N3Zn) is the zinc chloride salt form of Methyl green. Methyl green is a cationic or positive charged stain. Since it can binds selectively to the AT-rich regions of native DNA, it is mainly used for the DNA and RNA staining in the cell nuclei. Methyl Green, Zinc chloride Salt, as a CERTISTAIN® dry dye for DNA staining, it has many application: used as a tracking dye in acid buffers for electrophoresis; used to demonstrate DNA and RNA in tissues sections in the Methyl Green-Pyronin method; used as an exclusion viability stain for cells. In addition, it can also be used in fluorimetric assay and flow cytometry.
| | Indicator | Preparation of a methyl green zinc chloride double salt solution: 0.1 g of methyl green zinc chloride double salt in 100 mL of water. This solution is used as an acid-base indicator; at a pH of 0.1 to 2.3, the colour changes from yellow to blue. | | References | http://www.sigmaaldrich.com/catalog/product/sigma/67060?lang=zh®ion=CN
https://pubchem.ncbi.nlm.nih.gov/compound/3084002#section=Canonical-SMILES
https://en.wikipedia.org/wiki/Methyl_green
http://www.abcam.com/methyl-green-zinc-chloride-salt-ab146287.html
| | Chemical Properties | maroon to reddish-brown crystals | | Uses | Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently. | | Biological Activity | Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. at low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites. | | Purification Methods | Crystallise the dye from hot water. [Beilstein 13 IV 2286.] |
| | Methyl Green zinc chloride salt Preparation Products And Raw materials |
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