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| | Bo-Pro 1 Basic information |
| Product Name: | Bo-Pro 1 | | Synonyms: | BENZOTHIAZOLIUM, 3-METHYL-2-[[1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-PYRIDINYLIDENE]METHYL]-, DIIODIDE;3-METHYL-2-([1-[3-(TRIMETHYLAMMONIO)PROPYL]-4(1H)-PYRIDINYLIDENE]METHYL)-1,3-BENZOTHIAZOL-3-IUM DIIODIDE;BO-PRO-1 Iodide;Bo-Pro 1 | | CAS: | 157199-57-0 | | MF: | C20H27I2N3S | | MW: | 595.32 | | EINECS: | | | Product Categories: | | | Mol File: | 157199-57-0.mol |  |
| | Bo-Pro 1 Chemical Properties |
| solubility | Soluble in dimethyl sulfoxide | | form | Solid | | Appearance | yellow solution | | λmax | 462 nm (H2O/DNA) | | ex/em | 462/481 nm | | ε(extinction coefficient) | 58100 cm−1M−1 | | Φ(quantum yield) | 0.16 | | Biological Applications | Detecting nucleic
acids, cells, microorganisms, classifying/
counting megakaryocyte; counting erythroblasts,
sperms; nucleic acids amplification; nucleic acid
sequencing; as temperature sensor |
| | Bo-Pro 1 Usage And Synthesis |
| Description | BO-TAP-1 (also known as BO-PRO®-1) is a cyan fluorescent carbocyanine monomeric dye. BO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.
The bright fluorescence signal and low background make BO-TAP-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.
The dye is used to detect and count cells (megakaryocyte, erythroblasts, sperms, microorganisms, etc.) and bacteria, perform nucleic acid detection, sequencing, and amplification and as a temperature sensor. | | Chemical Properties | ex/em : 462/481 nm
Extinction coefficient (ε) : 58100 cm1M1
Quantum yield (Φ) : 0.16 | | Abs/Em Maxima | 454 (complex)/487 (complex) nm |
| | Bo-Pro 1 Preparation Products And Raw materials |
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