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| | 2-([1-(3-[[3-(Dimethyl(3-[4-[(3-methyl-1,3-benzothiazol-3-ium-2-yl)methylene]-1(4H)-pyridinyl]propyl)ammonio)propyl](dimethyl)ammonio]propyl)-4(1H)-pyridinylidene]methyl)-3-methyl-1,3-benzothiazol-3-ium tetraiodide Basic information |
| Product Name: | 2-([1-(3-[[3-(Dimethyl(3-[4-[(3-methyl-1,3-benzothiazol-3-ium-2-yl)methylene]-1(4H)-pyridinyl]propyl)ammonio)propyl](dimethyl)ammonio]propyl)-4(1H)-pyridinylidene]methyl)-3-methyl-1,3-benzothiazol-3-ium tetraiodide | | Synonyms: | Benzothiazolium, 2,2
0
-[1,3-propanediyl-
bis[(dimethyliminio)-3,1-propanediyl-1(4
h
)-pyridinyl-
4-ylidenemethylidyne]]bis[3-methyl-, Tetraiodide;BOBO 1;BOBO-1 IODIDE;BENZOTHIAZOLIUM, 2,2'-[1,3-PROPANEDIYLBIS[(DIMETHYLIMINIO)-3,1-PROPANEDIYL-1(4H)-PYRIDINYL-4-YLIDENEMETHYLIDYNE]]BIS[3-METHYL-, TETRAIODIDE;2-([1-(3-[[3-(Dimethyl(3-[4-[(3-methyl-1,3-benzothiazol-3-ium-2-yl)methylene]-1(4H)-pyridinyl]propyl)ammonio)propyl](dimethyl)ammonio]propyl)-4(1H)-pyridinylidene]methyl)-3-methyl-1,3-benzothiazol-3-ium tetraiodide | | CAS: | 169454-13-1 | | MF: | C41H54IN6S2+3 | | MW: | 821.95 | | EINECS: | | | Product Categories: | Cyanine | | Mol File: | 169454-13-1.mol | ![2-([1-(3-[[3-(Dimethyl(3-[4-[(3-methyl-1,3-benzothiazol-3-ium-2-yl)methylene]-1(4H)-pyridinyl]propyl)ammonio)propyl](dimethyl)ammonio]propyl)-4(1H)-pyridinylidene]methyl)-3-methyl-1,3-benzothiazol-3-ium tetraiodide Structure](CAS/GIF/169454-13-1.gif) |
| | 2-([1-(3-[[3-(Dimethyl(3-[4-[(3-methyl-1,3-benzothiazol-3-ium-2-yl)methylene]-1(4H)-pyridinyl]propyl)ammonio)propyl](dimethyl)ammonio]propyl)-4(1H)-pyridinylidene]methyl)-3-methyl-1,3-benzothiazol-3-ium tetraiodide Chemical Properties |
| Melting point | >250 ℃ | | solubility | Soluble in dimethyl sulfoxide | | form | powder | | color | Yellow-brown | | Appearance | orange solution | | λmax | 462 nm | | ex/em | 462/481 nm | | ε(extinction coefficient) | 113600 cm−1M−1 | | Φ(quantum yield) | 0.22 | | Biological Applications | Nucleic acid hybridization; detecting nucleic acids,cells,pathogens; counting embryoblasts; characterization of DNA/lipid complexes |
| | 2-([1-(3-[[3-(Dimethyl(3-[4-[(3-methyl-1,3-benzothiazol-3-ium-2-yl)methylene]-1(4H)-pyridinyl]propyl)ammonio)propyl](dimethyl)ammonio]propyl)-4(1H)-pyridinylidene]methyl)-3-methyl-1,3-benzothiazol-3-ium tetraiodide Usage And Synthesis |
| Description | BODi-1 (also known as BOBO®-1) is a cyan fluorescent carbocyanine dimeric dye. BODi-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.
The bright fluorescence signal and low background make BODi-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.
The dye is used to detect and count cells (megakaryocyte, erythroblasts, sperms, microorganisms, etc.), perform nucleic acid detection, characterizing DNA/lipid complexes (lipoplexes), and as a temperature sensor. | | Chemical Properties | ex/em: 462/481 nm
Extinction coefficient (ε): 113600 cm1M1
Quantum yield (Φ): 0.22 | | Abs/Em Maxima | 457 (complex)/488 (complex) nm |
| | 2-([1-(3-[[3-(Dimethyl(3-[4-[(3-methyl-1,3-benzothiazol-3-ium-2-yl)methylene]-1(4H)-pyridinyl]propyl)ammonio)propyl](dimethyl)ammonio]propyl)-4(1H)-pyridinylidene]methyl)-3-methyl-1,3-benzothiazol-3-ium tetraiodide Preparation Products And Raw materials |
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