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| | 4-Methylumbelliferyl oleate Basic information |
| | 4-Methylumbelliferyl oleate Chemical Properties |
| Melting point | 37-38 °C (lit.) | | Boiling point | 471.81°C (rough estimate) | | density | 1.023 | | refractive index | 1.4840 (estimate) | | Fp | 281.3℃ | | storage temp. | -20°C | | solubility | 2-methoxyethanol: soluble | | form | Solid | | color | Colorless to Pale Yellow Oil | | Appearance | Solid Powder | | BRN | 4887028 | | InChI | 1S/C28H40O4/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-27(29)31-24-19-20-25-23(2)21-28(30)32-26(25)22-24/h10-11,19-22H,3-9,12-18H2,1-2H3/b11-10- | | InChIKey | NKQFKJYKCVDLPT-KHPPLWFESA-N | | SMILES | CCCCCCCC\C=C/CCCCCCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1 | | CAS DataBase Reference | 18323-58-5(CAS DataBase Reference) |
| WGK Germany | 3 | | F | 8-10 | | HS Code | 29322090 | | Storage Class | 11 - Combustible Solids |
| | 4-Methylumbelliferyl oleate Usage And Synthesis |
| Description | 4-Methylumbelliferyl (4-MU) oleate is a fluorogenic substrate for acid and alkaline lipases. It is cleaved by lipases, liberating 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. | | Chemical Properties | Colourless Oil | | Uses | 4-Methylumbelliferyl oleate can act as Fatty acyl esters for use as fluorogenic substrates to continuously follow the hydrolytic activity of carboxylesterases, such as acid and alkaline lipases. | | Uses | Fluorogenic substrate for lipases. | | Uses | 4-Methylumbelliferyl oleate has been used:
- as a substrate to assay the activity of non-specific esterases
- to determine lipase activity
- as a substrate to evaluate pancreatic lipase inhibitory activity
| | References | [1] M L ARMSTRONG. Lipid metabolism in xanthomatous skin of hypercholesterolemic rabbits.[J]. American Journal of Pathology, 1986, 125 2: 339-348.
[2] BERNARD J MONCLA K M P. Oleate lipase activity in Gardnerella vaginalis and reconsideration of existing biotype schemes.[J]. ACS Applied Energy Materials, 2009: 78. DOI: 10.1186/1471-2180-9-78
[3] AKIHIRO OGAWA. Lactobacillus gasseri SBT2055 suppresses fatty acid release through enlargement of fat emulsion size in vitro and promotes fecal fat excretion in healthy Japanese subjects.[J]. Lipids in Health and Disease, 2015, 14: 20. DOI: 10.1186/s12944-015-0019-0 | | Excitation / Emission Maximum | 330 nm (pH 4.6), 370 nm (pH 7.4), 385 nm (pH 10.4)/445-454 nm |
| | 4-Methylumbelliferyl oleate Preparation Products And Raw materials |
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