|
|
| | D-Luciferin sodium salt Basic information |
| Product Name: | D-Luciferin sodium salt | | Synonyms: | (S)-2-(6-HYDROXY-2-BENZOTHIAZOLYL)-2-THIAZOLINE-4-CARBOXYLIC ACID SODIUM SALT;FIREFLY LUCIFERIN MONOSODIUM SALT MONOHYDRATE;FIREFLY LUCIFERIN SODIUM SALT;D-LUCIFERIN, MONOSODIUM SALT;D-LUCIFERIN SODIUM SALT;D(-)-LUCIFERIN SODIUM SALT MONOHYDRATE;D-LUCIFERIN SODIUM SALT MONOHYDRATE;D-(-)-2-[6'-HYDROXY-2'-BENZOTHIAZOLYL]DELTA-THIAZOLINE-4-CARBOXYLIC ACID SODIUM SALT | | CAS: | 103404-75-7 | | MF: | C11H9N2NaO3S2 | | MW: | 304.31 | | EINECS: | 600-430-4 | | Product Categories: | | | Mol File: | 103404-75-7.mol |  |
| | D-Luciferin sodium salt Chemical Properties |
| storage temp. | -20°C | | solubility | H2O: soluble | | form | lyophilized powder | | color | Yellow | | biological source | synthetic | | Water Solubility | H2O: soluble | | InChI | InChI=1/C11H8N2O3S2.Na.H/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;;/h1-3,7,14H,4H2,(H,15,16);;/t7-;;/s3 | | InChIKey | ZONJDNKUJJUQCK-DCFBZVEJNA-N | | SMILES | C([C@H]1CSC(C2=NC3C=CC(O)=CC=3S2)=N1)(=O)O.[NaH] |&1:1,r| | | CAS DataBase Reference | 103404-75-7(CAS DataBase Reference) |
| | D-Luciferin sodium salt Usage And Synthesis |
| Description | D-Luciferin is a chemiluminescent substrate of firefly luciferase. It produces light upon oxidative decarboxylation by luciferase in the presence of ATP. D-luciferin can be employed to assay the expression of the luciferase gene linked to a promoter of interest. Alternatively, D-luciferin and luciferase can be used to assess ATP availability in cellular or biochemical assays. | | Chemical Properties | Yellow powder | | Uses | D-Luciferin sodium salt is substrate for firefly luciferase, properties and use in assays of ATP and luciferase. | | Uses | Luciferin is used for luciferase assays and determination of ATP. | | Uses | D-Luciferin sodium salt has been used as a substrate in luciferinluciferase chemiluminescence assay to determine ATP levels. | | General Description | D-Luciferin is mainly produced in bioluminescent organisms. It can simply circulate across the blood testis barrier, blood brain barrier and the blood placenta barrier. This small molecule is sensitive to light and oxygen. D-Luciferin is also sensitive to moisture in its powder form. | | Biological Activity | D-Luciferin sodium salt is a firefly luciferase substrate (Km = ~2 μM). Used in much standard bioluminescence imaging (BLI) techniques, including monitoring tumor growth and investigating intracellular signaling activity in vitro and in vivo. Cell permeable. Emission maximum = 562 nm.
 | | Synthesis | Produced by the condensation of resorcinol with phthalic anhydride in the presence of zinc chloride or sulfuric acid, which is then converted to the sodium salt.
| | storage | -20°C | | References | [1] LEMBERT N. Firefly luciferase can use L-luciferin to produce light.[J]. Biochemical Journal, 1996, 317 ( Pt 1): 273-277. DOI: 10.1042/bj3170273 |
| | D-Luciferin sodium salt Preparation Products And Raw materials |
|