羟基法舒地尔 CAS#: 105628-72-6

羟基法舒地尔

中文名称	羟基法舒地尔
中文同义词	法舒地尔杂质L;5-[(六氢-1H-1,4-二氮杂卓-1-基)磺酰基]-1(2H)-异喹啉酮;1 - [(1,2-二氢-1-氧代-5-异喹啉基)磺酰基]六氢-1H-1,4-二氮杂盐酸盐;5-((1,4-二氮杂卓-1-基)磺酰基)异喹啉-1(2H)-酮;5-((1,4-二氮杂环庚烷-1-基)磺酰基)异喹啉-1(2H)-酮;法舒地尔23;1-[(1,2-二氢-1-氧代-5-异喹啉YL)磺酰基]六氢-1H-1,4-二氮杂卓;羟基法舒地尔,10 MM DMSO 溶液
英文名称	1-[(1,2-DIHYDRO-1-OXO-5-ISOQUINOLINYL)SULFONYL]HEXAHYDRO-1H-1,4-DIAZEPINE
英文同义词	5-(1,4-diazepane-1-sulfonyl)-1,2-dihydroisoquinolin-1-one;HYDROXYFASUDIL;HA-1100;HA 1100;HA1100;1-[(1,2-DIHYDRO-1-OXO-5-ISOQUINOLINYL)SULFONYL]HEXAHYDRO-1H-1,4-DIAZEPINE;1H-1,4-Diazepine, hexahydro-1-((1,2-dihydro-1-oxo-5-isoquinolinyl)sulfonyl)-;Ha 1100;Hexahydro-1-((1,2-dihydro-1-oxo-5-isoquinolinyl)sulfonyl)-1H-1,4-diazepine;1-[(1,2-Dihydro-1-oxo-5-isoquinolinyl)sulfonyl]hexahydro-1H-1,4-diazepinehydrochloride;5-((1,4-diazepan-1-yl)sulfonyl)isoquinolin-1-ol
CAS号	105628-72-6
分子式	C14H17N3O3S
分子量	307.37
EINECS号
相关类别	细胞周期;Angiogenesis and Metastasis;Intracellular signaling
Mol文件	105628-72-6.mol
结构式

羟基法舒地尔性质

储存条件	Sealed in dry,Store in freezer, under -20°C
溶解度	溶于二甲基亚砜
形态	固体
颜色	白色至米白色
InChI	InChI=1S/C14H17N3O3S/c18-14-12-3-1-4-13(11(12)5-7-16-14)21(19,20)17-9-2-6-15-8-10-17/h1,3-5,7,15H,2,6,8-10H2,(H,16,18)
InChIKey	ZAVGJDAFCZAWSZ-UHFFFAOYSA-N
SMILES	C1(=O)C2=C(C(S(N3CCCNCC3)(=O)=O)=CC=C2)C=CN1

羟基法舒地尔用途与合成方法

生物活性

Hydroxyfasudil 是一种 ROCK 抑制剂，可抑制 ROCK1 和 ROCK2 的活性，IC50 值分别为 0.73 和 0.72 μM。

靶点

ROCK2

0.72 μM (IC ₅₀ )

ROCK1

0.73 μM (IC ₅₀ )

PKA

37 μM (IC ₅₀ )

体外研究

Hydroxyfasudil is a ROCK inhibitor, with IC ₅₀ s of 0.73 and 0.72 μM for ROCK1 and ROCK2, respectively. Hydroxyfasudil also less potently inhibits PKA, with an IC ₅₀ of 37 μM, 50-fold higher than those of the ROCKs. Hydroxyfasudil increases eNOS mRNA levels, with an EC ₅₀ value of 0.8 ± 0.3 μM. Hydroxyfasudil (0-100 μM) concentration-dependently increases eNOS activity and stimulates NO production in human aortic endothelial cells (HAEC). Hydroxyfasudil (10 μM) increases the half-life of eNOS mRNA from 13 to 16 hours, but does not affect eNOS promoter activity at concentrations from 0.1 to 100 μM.

体内研究

Hydroxyfasudil (10 mg/kg, i.p.) significantly increases both the average and maximal voided volumes in SD rats. Hydroxyfasudil also significantly decreases the maximal detrusor pressure. Hydroxyfasudil (3 mg/kg, i.p) inhibits hypercontractility induced by norepinephrine in spontaneously hypertensive rats (SHRs). Furthermore, Hydroxyfasudil (3, 10 mg/kg, i.p) significantly ameliorates decreased penile cGMP contents in rats.

安全信息

毒性	mouse,LD50,unreported,145mg/kg (145mg/kg),United States Patent Document. Vol. #4678783,

羟基法舒地尔