2',7'-二氯荧光素二乙酸酯
| 中文名称 | 2',7'-二氯荧光素二乙酸酯 |
|---|---|
| 中文同义词 | 2,7-二氯二氢荧光素二乙酸;2,7-二氯二氢代荧光黄二醋酸;DCFH-DA 活性氧ROS荧光探针;2-(3,6-二乙酰氧基-2,7-二氯-9H-氧杂蒽-9-基)苯甲酸;活性氧ROS荧光探针/ 二氯二氢荧光素-乙酰乙酸酯;2',7'-二氯荧光素二乙酸酯 50MG;2,7-二氯二氢荧光素二乙酸酯;2,7-螢光素二醋酸酯 |
| 英文名称 | 2′,7′-Dichlorodihydrofluorescein Diacetate |
| 英文同义词 | 187263;2,7-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem;2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate;2',7'-DICHLOROFLUORESCIN DIACETATE, FOR FLUORESCENCE;2',7'-Dichlorodihydrofluoresceinediacetate;Dichlorofluorescin diacetate;2'',7''-Dichlorofluorescein diac;2-(2,7-Dichloro-3,6-diacetoxy-9H-xanthen-9-yl)benzoic acid |
| CAS号 | 4091-99-0 |
| 分子式 | C24H16Cl2O7 |
| 分子量 | 487.29 |
| EINECS号 | 685-843-8 |
| 相关类别 | 荧光探针，标记，颗粒和染色;生化试剂-植物激素及核酸类;生化试剂;通用生化试剂-生物染色剂与化学指示剂;化学试剂 |
| Mol文件 | 4091-99-0.mol |
| 结构式 |  |
2',7'-二氯荧光素二乙酸酯 性质
| 熔点 | 209-210 °C(lit.) |
|---|---|
| 沸点 | 593.5±50.0 °C(Predicted) |
| 密度 | 1.463±0.06 g/cm3(Predicted) |
| 储存条件 | -20°C |
| 溶解度 | 在DMF中可溶 |
| 酸度系数(pKa) | 3.79±0.36(Predicted) |
| 形态 | 结晶固体 |
| 颜色 | 白色至米白色 |
| 外观 | colorless solid |
| BRN | 4610273 |
| 稳定性 | 感光 |
| InChI | InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30) |
| InChIKey | PXEZTIWVRVSYOK-UHFFFAOYSA-N |
| SMILES | C(O)(=O)C1=CC=CC=C1C1C2=C(C=C(OC(C)=O)C(Cl)=C2)OC2=C1C=C(Cl)C(OC(C)=O)=C2 |
| EPA化学物质信息 | 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0) |
2',7'-二氯荧光素二乙酸酯是一种荧光探针，作为一种非极性化合物，能够迅速扩散到细胞内，细胞内的酯酶将其水解为非荧光的二氯荧光素（DCFH），从而使探针被装载到细胞内。
H2DCFDA (DCFH-DA, 2',7'-Dichlorodihydrofluorescein diacetate) 是一种可渗透细胞的探针，可用于检测细胞内 reactive oxygen species (ROS) 的产生。| Target | Value |
|
ROS
() |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA.
安全信息
| 危险品标志 | Xi |
|---|---|
| 危险类别码 | 36/37/38 |
| 安全说明 | 22-24/25-26 |
| WGK Germany | 3 |
| F | 10-21 |
| TSCA | TSCA listed |
| 海关编码 | 29329990 |
| 存储类别 | 11 - 可燃固体 |