- EPPS
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- $30.00
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2025-06-27
- CAS:16052-06-5
- Min. Order: 50KG
- Purity: 99%
- Supply Ability: 500000kg
- HEPPS
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- $32.00
-
2025-04-15
- CAS:16052-06-5
- Min. Order: 1kg
- Purity: 99%
- Supply Ability: 5000kg/week
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| | HEPPS Chemical Properties |
| Melting point | 237-239 °C(lit.) | | density | 1.2684 (rough estimate) | | refractive index | 1.6800 (estimate) | | storage temp. | Sealed in dry,Room Temperature | | solubility | H2O: 1 M at 20 °C, clear, colorless | | form | Powder/Solid | | color | White | | PH | 5.0-6.5 (25℃, 0.1M in H2O) | | pka | 8.0(at 25℃) | | PH Range | 7.3 - 8.7 | | Water Solubility | Soluble in water. 25.2 g/L (20°C) | | λmax | λ: 260 nm Amax: 0.055
λ: 280 nm Amax: 0.045 | | BRN | 3957385 | | Major Application | diagnostic assay manufacturing | | InChI | 1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15) | | InChIKey | OWXMKDGYPWMGEB-UHFFFAOYSA-N | | SMILES | OCCN1CCN(CCCS(O)(=O)=O)CC1 | | CAS DataBase Reference | 16052-06-5(CAS DataBase Reference) | | EPA Substance Registry System | 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)- (16052-06-5) | | Absorption | ≤0.1 at 260 in H2O at 1M
≤0.1 at 280 in H2O at 1M |
| Hazard Codes | Xi | | Risk Statements | 36/37/38-22 | | Safety Statements | 24/25-36-26 | | WGK Germany | 3 | | RTECS | TM2649130 | | TSCA | TSCA listed | | HazardClass | IRRITANT | | HS Code | 29335995 | | Storage Class | 11 - Combustible Solids | | Toxicity | quail,LD50,oral,> 316mg/kg (316mg/kg),Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982. |
| | HEPPS Usage And Synthesis |
| Chemical Properties | white crystalline powder | | Uses | HEPPS (EPPS) is a commonly used Good's buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry. HEPPS is a biological buffer with a useful pH range of 7.3-8.7 and a pKa=8.0 at 25°C. Research on mice with Alzheimer's disease-like amyloid beta plaques has shown that HEPPS can cause the plaques to break up, reversing some of the symptoms in the mice. HEPPS was reported to dissociate amyloid beta oligomers in patients' plasma samples enabling blood diagnosis of Alzheimer's disease. | | Definition | ChEBI: HEPPS is a N-(sulfoalkyl)piperazine and a N-(2-hydroxyethyl)piperazine. | | General Description |
HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. Its mechanism of action is rooted in its ability to maintain a stable pH environment, which is crucial for various enzymatic reactions and protein studies. HEPPS is especially favoured for its buffering capacity in the physiological pH range, typically between pH 6.8 and 8.2, making it ideal for biological assays and cell culture experiments. This compound is extensively utilized in preparing cell culture media, where maintaining a consistent pH is essential for cell viability and growth. Additionally, HEPPS is employed in biochemical assays, such as enzyme kinetics studies, where precise pH control is necessary to accurately measure enzymatic activity. Furthermore, HEPPS has found applications in molecular biology techniques, including nucleic acid purification, PCR (polymerase chain reaction), and protein analysis by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Its compatibility with various biological systems and stability under physiological conditions make HEPPS a valuable tool in research laboratories for maintaining optimal pH conditions and ensuring reliable experimental outcomes in various biochemical and molecular biology studies.
| | Flammability and Explosibility | Not classified |
| | HEPPS Preparation Products And Raw materials |
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