- neuraminidase
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- $42.00
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2023-08-03
- CAS:9001-67-6
- Min. Order: 10KU
- Purity: ≥400U/mg
- Supply Ability: 1000ku
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| | Neuraminidase Basic information |
| Product Name: | Neuraminidase | | Synonyms: | α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli;α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli;EC 3.2.1.18;EC 3.2.1.18 TYPE II;EC 3.2.1.18 TYPE III;EC 3.2.1.18 TYPE V;EC 3.2.1.18 TYPE VI;EC 3.2.1.18 TYPE VI-A | | CAS: | 9001-67-6 | | MF: | NULL | | MW: | 0 | | EINECS: | 232-624-6 | | Product Categories: | | | Mol File: | Mol File | ![Neuraminidase Structure]() |
| | Neuraminidase Chemical Properties |
| density | 1.00 g/mL at 20 °C | | storage temp. | 2-8°C | | form | lyophilized powder | | color | white | | biological source | Vibrio cholerae | | Water Solubility | Soluble in water or 10mM phosphate buffer, pH 6.0 | | Specific Activity | 8-24units/mg protein (Lowry, using NAN-lactose) | | EPA Substance Registry System | Neuraminidase (9001-67-6) |
| Safety Statements | 22-24/25 | | RIDADR | UN3373 - class 6.2 Biological substance, Category B | | WGK Germany | 3 | | RTECS | QQ3450000 | | F | 3-10 | | TSCA | TSCA listed | | Storage Class | 11 - Combustible Solids | | Hazard Classifications | Resp. Sens. 1 |
| | Neuraminidase Usage And Synthesis |
| Description | Neuraminidase, also known as sialidase, is a family of glycosidases (EC 3.2.1.18) capable of hydrolysing terminal sialic acid residues on glycolipids, oligosaccharides and glycoproteins; this constitutes the first step in their degradation. Neuraminidases have been identified in a range of organisms, including viruses, protozoa, bacteria, fungi and vertebrates. The best-known neuraminidase is viral neuraminidase (viNEU), which is a drug target for preventing the spread of influenza infection. In mammals, four types of neuraminidase have been characterised: NEU1, NEU2, NEU3 and NEU4. | | Uses | Neuraminidase is used as a hydrolytic enzyme that removes sialic acid from mucoproteins, used in a study to assess binding with human T lymphocytes in sheep pretreated with neuraminidase. It has also been used in a study to investigate the effect of bile salts on the action of hydrolysis by neuraminidase. | | Uses | Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein. | | Uses | Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells. | | General Description | Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release. | | Biochem/physiol Actions | Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells. | | References | [1] Gatt, S, et al. “Effect of Bile Salts on the Hydrolysis of Gangliosides, Glycoproteins and Neuraminyl-Lactose by the Neuraminidase of Clostridium Perfringens.” Biochemical Journal, 193 1, 1981, pp. 267–73, https://doi.org/10.1042/bj1930267.
[2] Deman, J J, et al. “A Study on the Mechanism of Intercellular Adhesion. Effects of Neuraminidase, Calcium, and Trypsin on the Aggregation of Suspended HeLa Cells.” Journal of Cell Biology, 60 3, 1974, pp. 641–52, https://doi.org/10.1083/jcb.60.3.641.
[3] Weiner, Michael S., et al. “Enhanced Binding of Neuraminidase-Treated Sheep Erythrocytes to Human T Lymphocytes.” Blood, 42 6 1, 1973, pp. 939–46, https://doi.org/10.1182/BLOOD.V42.6.939.939. |
| | Neuraminidase Preparation Products And Raw materials |
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