Product Details
| Product Name: 3X FLAG PEPTIDE | Min. Order: 1KG |
| Purity: 99% | Supply Ability: 1T |
| Release date: 2025/10/22 |
| Product Name: | 3X FLAG PEPTIDE |
| Synonyms: | 3X FLAG(R) Peptide;3X FLAG peptide,Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys,MDYKDHDGDYKDHDIDYKDDDDK;MET-ASP-TYR-LYS-ASP-HIS-ASP-GLY-ASP-TYR-LYS-ASP-HIS-ASP-ILE-ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS;3X FLAG PEPTIDE;MDYKDHDGDYKDHDIDYKDDDDK;L-Lysine, L-methionyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartylglycyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartyl-L-isoleucyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-;Flag Peptide trifluoroacetate salt;3×FLAG peptide |
| CAS: | 402750-12-3 |
| MF: | C120H169N31O49S |
| MW: | 2861.9 |
| EINECS: | |
| Product Categories: | EpitopeTags |
| Mol File: | 402750-12-3.mol |
 | |
| 3X FLAG PEPTIDE Chemical Properties |
| Boiling point | 2863.6±65.0 °C(Predicted) |
| density | 1.458±0.06 g/cm3(Predicted) |
| storage temp. | 2-8°C |
| solubility | ≥25mg/ml in TBS (0.5M Tris-HCl, pH 7.4, with 1M NaCl), 1X TBS as recommended buffer. |
| form | lyophilized powder |
| color | White to off-white |
| Sequence | Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys |
| InChIKey | SNXSKDGBSPFDDR-SHFSSAJFNA-N |
| Safety Information |
| WGK Germany | 3 |
| MSDS Information |
| Provider | Language |
|---|---|
| SigmaAldrich | English |
| 3X FLAG PEPTIDE Usage And Synthesis |
| Uses | 3X FLAG peptides are FLAG-tagged peptides containing three repeats of the Asp-Tyr-Lys-Xaa-Xaa-Asp motif. 3X FLAG peptide can be used for protein separation and purification, and competitive elution with target proteins. |
| Biological Activity | the dykddddk-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1. the dykddddk peptide binds to the antibody m1. whether binding is calcium-dependent manner2 or –independent3 remains controversial. a disadvantage of the system is that the monoclonalantibody purification matrix is not as stable as others. in general, small tags can be detected with specific monoclonal antibodies.to improve the detection of the dykddddk tag the 3x (dykddddk) system has been developed. this threetandem dykddddk epitope is hydrophilic, 22-amino-acids long and can detect up to 10 fmol of expressed fusion protein. the dykddddk-tagged maltodextrin-binding protein of pyrococcus furiosus has been crystallized4 and the quality of the crystals was very similar to that of crystals of untagged protein.finally, the dykddddk-tag can be removed by treatment with enterokinase, which is specific for the five c-terminal amino acids of the peptide sequence5. |
| references | 1. hopp tp, prickett ks, price vl, libby rt, march cj, ceretti dp, urdal dl, conlon pj (1988) a short polypeptide marker sequence useful for recombinant protein identification and purification. bio/technology 6:1204–1210.2. hopp tp, gallis b, prikett ks (1996) metal-binding properties of a calcium dependent monoclonal antibody. mol immunol 33:601–608.3. einhauer a, jungbauer a (2000) kinetics and thermodynamical properties of the monoclonal antibody m1 directed against the flag peptide. 20th international symposium on the separation of proteins, peptides, and polynucleotides (isppp). lublijana, slovenia, november 5–8, 2000.4. bucher mh, evdokimov ag, waugh ds (2002) differential effects of short affinity tags on the crystallization of pyrococcus furiosus maltodextrin-binding protein. biol cryst 58:392–397.5. maroux s, baratti j, desnuelle p (1971) purification and specificity of procine enterokinase. j biol chem 246:5031–5039. |
Company Profile Introduction
Shandong Hanjiang Chemical Co., Ltd. is located in Zibo City, Shandong Province, China.
It is a biotechnology enterprise engaged in the R&D, production and sales of drugs, steroids, peptides, raw materials, vitamins, food additives, animal and plant extracts, cosmetics, pharmaceutical raw materials and intermediates. The company has complete experimental facilities. Advanced testing instruments ensure the stability of product quality from all aspects. The company has a complete sales service system, and its products are exported to countries all over the world. It has won a good international reputation for its excellent quality and excellent service. The company has been adhering to the basic principles of "integrity, quality, service, and win-win" to serve customers, and constantly strict requirements,
Advantages:
1. Samples are provided for test;
2. Good quality products;
3. 100% secure delivery.
4. secure your money, lower the risk
Payment ways: Western Union, Moneygram, Bitcoin, T/T
Delivery: EMS/ EUB/ USPS/ UPS/ TNT/ FEDEX/ DHL etc
Packaging:safe and discreet packaging
Our company Superiority
1 Best service, high quality and reasonable price
2. It's customers' right to choose the package (EMS, DHL, FedEx, UPS);
3. It's customers' right to choose the packing way for his products from many recent effective packing ways
4. Specials are possible when client's order is big enough, including the discount policy;
5.Our company promise to deliver clients' package to his hands safely, or we'll cover the total loss and reship in time;
Our company supply high quality chemical products with the most competitive prices.
If have any need or question, contact us freely.
Hope we can have chances to build good long-term cooperation.
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