7-BFC NEW
| Price | $40 | $64 | $135 |
| Package | 5mg | 10mg | 25mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-12-05 |
Product Details
| Product Name: 7-BFC | CAS No.: 220001-53-6 |
| Purity: 99.71% | Supply Ability: 10g |
| Release date: 2025/12/05 |
Product Introduction
Bioactivity
| Name | 7-BFC |
| Description | 7-BFC (7-Benzyloxy-4-(trifluoromethyl)coumarin) is a coumarin-like fluorescent substrate that serves as a biomarker for cytochrome P 450 and can be used to study CYP isoforms and cytochrome P 450 metabolism. |
| In vitro | I. CYP activity detection 1. Material preparation: 1) 7-BFC solution: usually prepared as a 1-10 µM solution, dissolved in an appropriate solvent (such as DMSO or PBS). 2) Enzyme source: CYP subtype (such as CYP3A4, CYP2D6, etc.) expression system, or use human liver microsomes. 3) Reaction buffer: usually use phosphate buffer (pH 7.4) containing NADPH to simulate the in vivo metabolic environment. 4) Fluorescence detection equipment: such as fluorescence spectrophotometer, excitation wavelength 405 nm, emission wavelength 460 nm (specific wavelength depends on the experimental setting). 2. Steps: 1) Prepare the reaction system: mix the 7-BFC solution with CYP subtypes or liver microsomes, and add NADPH as an electron donor. 2) Reaction incubation: Incubate the reaction system at 37°C for 15-30 minutes to allow 7-BFC to undergo metabolic reactions and produce fluorescence. 3) Fluorescence detection: Use a fluorescence spectrophotometer to detect the fluorescence intensity of the product, usually setting the excitation wavelength to 405 nm and the emission wavelength to 460 nm. 4) Data analysis: By measuring the changes in fluorescence intensity, the activity of cytochrome P450 and its metabolic efficiency on 7-BFC can be evaluated. II. CYP subtype-specific detection 1. Material preparation: 1) CYP subtype inhibitors: such as ketoconazole (an inhibitor of CYP3A4), or inhibitors of specific subtypes. 2) Metabolite analysis: Metabolites can be further analyzed by techniques such as high-performance liquid chromatography (HPLC). 3. Steps: 1) Set up experimental groups: Add different CYP inhibitors to identify the metabolic effects of specific CYP subtypes on 7-BFC. 2) Fluorescence detection and data analysis: Compare the fluorescence signal intensity of different inhibitors and untreated samples to further confirm the metabolic characteristics of specific CYP subtypes. Notes: 1) Reaction conditions: The choice of temperature, pH and buffer will affect the efficiency of the reaction and should be optimized according to the experimental requirements. 2) Solvent effect: When dissolving 7-BFC, a solvent that has no effect on the experimental system should be selected to avoid inhibition of CYP activity. 3) Fluorescence stability: The fluorescence signal of 7-BFC is relatively stable, but long-term exposure to strong light should be avoided. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 55 mg/mL (171.74 mM), Sonication is recommended. |
| Keywords | Y0400031 | Y040 0031 | Cytochrome P450 |
| Inhibitors Related | Tebuconazole | 1-Ethynylnaphthalene | Diflubenzuron | Naringin dihydrochalcone | Apigenin | Fenofibrate | 1-Aminobenzotriazole | Naringin | Naringenin | Tauroursodeoxycholate | Chlorzoxazone | Ticagrelor |
| Related Compound Libraries | Bioactive Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, USA
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