Hydroxyphenyl Fluorescein NEW
| Price | $497 | Get Latest Price |
| Package | 1mg | 5mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-10-03 |
Product Details
| Product Name: Hydroxyphenyl Fluorescein | CAS No.: 359010-69-8 |
| Supply Ability: 10g | Release date: 2025/10/03 |
Product Introduction
Bioactivity
| Name | Hydroxyphenyl Fluorescein |
| Description | Hydroxyphenyl Fluorescein (HPF) is a cell-permeable fluorescent dye for the detection of highly reactive oxygen species with little or no intrinsic fluorescence.Hydroxyphenyl Fluorescein can be used in fluorescent enzyme labeling and flow cytometry, and can be used to Neurodegeneration and aging. Fluorescence wavelength: Ex/Em = 490/515 nm. |
| Cell Research | Operation steps: a. Preparation of stock solution: Prepare 10 mM HPF with DMSO; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) b. Preparation of HPF working solution Dilute the stock solution and dissolve it in serum-free cell culture medium or PBS to prepare 1-10 μM HPF working solution; (select the appropriate working solution concentration according to experimental requirements and prepare it for immediate use) c. Cell staining 1. Suspended cells (6-well plate) a. Centrifuge at 1000 g for 3-5 minutes at 4℃, and then discard the supernatant. Wash twice with PBS for 5 minutes each. The cell density is 1×106/mL; b. Add 1 mL of working solution and incubate at room temperature for 15 minutes; c. Centrifuge at 4℃ and 400 g for 3-4 minutes, and discard the supernatant; d. Wash twice with PBS, 5 minutes each time; e. Resuspend cells with serum-free cell culture medium or PBS. Observe with fluorescence microscope or flow cytometer. 2. Adherent cells a. Culture adherent cells on sterile coverslips; b. Remove coverslips from culture medium and aspirate excess culture medium; c. Add 100 μL working solution, shake gently to completely cover cells, and incubate at room temperature for 15 minutes; d. Wash twice with culture medium, 5 minutes each time. Observe with fluorescence microscope or flow cytometer. |
| Storage | store at low temperature,keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 10 mg/mL (23.56 mM), Sonication is recommended. DMF : 8 mg/mL (18.85 mM), Sonication is recommended. Ethanol : 8 mg/mL (18.85 mM), Sonication is recommended. |
| Inhibitors Related | Ethoxyquin | Cysteamine hydrochloride | Acetylcysteine | Imeglimin hydrochloride | L-Ascorbic acid | Hesperidin | Glucosamine | Gallic Acid Monohydrate | L-Cystine | Coenzyme Q10 | L-Ascorbic acid sodium salt | Sodium 2-oxopropanoate |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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