Probucol Impurity 26 NEW

Probucol Impurity 26；64953-47-5 

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WhatsAPP: +86 17386083646
E-mail: anna@molcoo.com

Product Information

• Product Code：P063026

• English Name：Probucol Impurity 26

• English Alias：6,6'-disulfanediylbis(2,4-di-tert-butylphenol)

• CAS No.：64953-47-5

• Molecular Formula：C28H42O2S2

• Molecular Weight：474.76

Advantages

• High-Purity Guarantee：Confirmed by HPLC (≥99.0%) and verified through multiple methods including NMR, HRMS, and elemental analysis, providing a reliable standard for Probucol impurity analysis.

• Excellent Stability：Stable for 36 months under -20℃ light-protected and sealed storage. The degradation rate is less than 0.3% within 6 months in methanol - acetonitrile mixture, ensuring good repeatability of experimental data and meeting long-term research needs.

Applications

• Quality Control Testing：Used for UPLC-MS/MS detection of Impurity 26 in Probucol API and formulations. Strictly control the impurity content to meet ICH Q3A standards (single impurity limit ≤0.1%) and ensure drug quality and safety.

• Process Optimization Research：Monitor the formation pathway of this impurity during Probucol synthesis. By adjusting parameters such as condensation reaction temperature (e.g., 80 - 90℃), reaction time, and reactant ratio, the generation of impurities can be reduced by more than 35%.

• Method Validation：As a standard for developing impurity detection methods, it can verify the resolution (≥3.0) and limit of detection (0.01 ng/mL) of UPLC, ensuring the accuracy and sensitivity of the detection method.

Background Description

Research Status

• Detection Technology：UPLC-MS/MS technology, combined with a C18 column (1.7μm) and gradient elution with 0.1% formic acid - acetonitrile, achieves impurity separation within 10 minutes, with a detection limit as low as 0.003 ng/mL for high-precision trace impurity detection.

• Formation Mechanism：Studies show that this impurity is formed by the coupling reaction of 2,4-di-tert-butylphenol under the action of an oxidant (such as hydrogen peroxide) and treatment with a thio-reagent (such as sodium sulfide). Optimizing the dosage of oxidant and thio-reagent and the reaction pH can effectively inhibit side reactions.

• Safety Evaluation：In vitro cytotoxicity experiments show that the IC₅₀ of this impurity against HepG2 cells is 192.6 μM (Probucol IC₅₀ = 10.3 μM). Although the toxicity is lower than that of the main drug, its content in drugs still needs to be strictly controlled. Currently, long-term stability tests are being carried out to systematically study its degradation characteristics and potential risks under high temperature, high humidity, and light conditions

NOTE！

We can also customize related analogues and modified peptides including HPLC, MS, 1H-NMR, MS, HPLC, IR, UV, COA, MSDS.
This product is intended for laboratory use only!

WhatsAPP: +86 17386083646
E-mail: anna@molcoo.com

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The Molcoo Laboratory added drug impurity reference standards, including Baricitinib, Piperazine, Benzylpenicillin, Tranilast and multiple N-Nitroso drug impurities! Now available for immediate delivery!