Red-HyP-1 NEW
| Price | $195 | $483 | $692 |
| Package | 1mg | 5mg | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-06-17 |
Product Details
| Product Name: Red-HyP-1 | Purity: 96.69% |
| Supply Ability: 10g | Release date: 2025/06/17 |
Product Introduction
Bioactivity
| Name | Red-HyP-1 |
| Description | Red-HyP-1 is a red dye that can be used for in vivo hypoxia imaging. The maximum emission maximum of Red-HyP-1 is 798 nm. |
| Animal Research | Instructions 1. Solution preparation Stock solution: Dissolve Red-HyP-1 in anhydrous DMSO or DMF to prepare a 1–10 mM stock solution. Working solution: Dilute to a working concentration of 1–10 µM using an experimental buffer (e.g., PBS, pH 7.4). 2. Experimental steps In vitro experiments 1) Add Red-HyP-1 to the sample solution (e.g., cell culture medium or biological buffer). 2) Incubate for 30 minutes to 1 hour at 37°C, away from light. 3) Detect fluorescence signal: Excitation wavelength: 720–750 nm Emission wavelength: 798 nm In vivo hypoxia imaging 1. Inject Red-HyP-1 into experimental animals (e.g., tail vein injection, the specific dose depends on the experimental design, usually 1–5 mg/kg). Wait 30 minutes to 1 hour after injection and detect using a fluorescence imager. 2. Signal detection equipment Use near-infrared fluorescence imaging system or fluorescence microscope to record the signal. 3. Calibration and control 1) Control experiment: Set up control groups with normal oxygen concentration (such as 20% O₂) and low oxygen concentration (such as 1% O₂). Verify whether the signal is related to hypoxia. 2) Standard curve: Use samples under known oxygen concentration gradient to establish the relationship curve between fluorescence signal and oxygen concentration. Notes 1) Photosensitivity: Red-HyP-1 is sensitive to light. Avoid strong light exposure throughout the experiment to prevent fluorescence signal attenuation. 2) Storage conditions: Red-HyP-1 should be stored at -20°C in the dark and avoid repeated freezing and thawing. 3) Dissolution stability: Ensure that the solution is fully dissolved before use; if necessary, undissolved particles can be removed by low-speed centrifugation. |
| In vitro | Under hypoxic conditions, CYP450 enzymes react with the N-oxide group, undergoing two-electron reduction to yield the uncapped dye (Red-HyP-1) which results in a red-shifted emission. HyP-1 has an absorbance and emission maxima at 670 and 697 nm, respectively, while red-Hyp-1 (the turned over product) has an absorbance and emission maxima at 760 and 798 nm. [1] |
| Storage | keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Keywords | RedHyP1 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
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