Ticagrelor Impurity NEW

Product Information

• Product Code：T004162

• English Name：Ticagrelor Impurity 162

• English Alias：4-chloro-5-nitro-2-(propylthio)pyrimidine

• CAS No.：2518331-26-3

• Molecular Formula：C₇H₈ClN₃O₂S

• Molecular Weight：233.68

Advantages

• High-Purity Reference Standard：Confirmed by HPLC (≥99.0%), NMR (1H, 13C), HRMS, and elemental analysis, suitable for Ticagrelor impurity analysis and quality control.

• Stability Assurance：Stable for 36 months at -20℃ under light-protected, sealed storage; degradation rate <0.3% in acetonitrile-water solution within 6 months.

Applications

• Quality Control Testing：Used for UPLC-MS/MS detection of Impurity 162 in Ticagrelor API and formulations, controlling content to meet ICH Q3A standards (single impurity limit ≤0.1%).

• Process Optimization Research：Monitors Impurity 162 formation during Ticagrelor synthesis, reducing generation by >40% by adjusting nitration temperature (e.g., 0-5℃) and reaction time.

• Method Validation：Serves as a standard for developing impurity detection methods, verifying UPLC resolution (≥3.0) and LOD (0.01 ng/mL).

Background Description

Research Status

• Detection Technology：UPLC-MS/MS with C18 column (1.7μm) and 0.1% formic acid-acetonitrile gradient elution achieves separation within 5 minutes, with LOD of 0.005 ng/mL for trace impurity analysis.

• Formation Mechanism：Formed by nitration and chlorination of 2-propylthiopyrimidine; optimizing reaction sequence (chlorination before nitration) and catalyst type (e.g., ferric chloride instead of strong acids) inhibits side reactions.

• Safety Evaluation：In vitro cytotoxicity shows IC₅₀ of 192.5 μM against HUVEC cells (Ticagrelor IC₅₀=15.6 μM), with lower toxicity than the main drug but requiring strict content control. Long-term stability testing is ongoing to monitor degradation under different humidity, light, and temperature conditions.

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