Tryptophan EP Impurity K NEW

Tryptophan EP Impurity K

Product Information

• Product Code：T057011

• English Name：Tryptophan EP Impurity K

• English Alias：(S)-3-(2-((1H-indol-3-yl)methyl)-1H-indol-3-yl)-2-aminopropanoic acid

• CAS No.：149724-31-2

• Molecular Formula：C₂₀H₁₉N₃O₂

• Molecular Weight：333.38

Advantages

• High-Purity Reference Standard：Confirmed by HPLC (≥99.0%), NMR (1H, 13C), HRMS, and elemental analysis, compliant with EP (European Pharmacopoeia) impurity standards for Tryptophan quality control.

• Stability Assurance：Stable for 36 months at -20℃ under light-protected, sealed storage; degradation rate <0.3% in aqueous solution (pH 5-7) within 6 months.

Applications

• Quality Control Testing：Used for HPLC and LC-MS detection of EP Impurity K in Tryptophan API and formulations, controlling content to meet EP standards (≤0.1%).

• Process Optimization Research：Monitors impurity formation during Tryptophan synthesis, reducing generation by >50% by adjusting condensation temperature (e.g., 70-80℃) and reaction time.

• Method Validation：Serves as an EP standard for developing impurity detection methods, verifying HPLC resolution (≥2.5) and LOD (0.01 ng/mL).

Background Description

Research Status

• Detection Technology：UPLC-MS/MS with C18 column (1.7μm) and 0.1% formic acid-acetonitrile gradient elution achieves separation within 3.5 minutes, with LOD of 0.003 ng/mL for EP-compliant trace analysis.

• Formation Mechanism：Formed by reaction of 3-indolecarboxaldehyde with Tryptophan under acidic conditions (e.g., acetic acid catalysis); optimizing catalyst dosage and solvent polarity (e.g., ethanol-water system) inhibits side reactions.

• Safety Evaluation：In vitro cytotoxicity shows IC₅₀ of 178.6 μM against C2C12 myoblasts (Tryptophan IC₅₀=21.3 μM), with low toxicity but requiring ≤0.1% limit. Long-term stability testing is ongoing to monitor degradation under different pH conditions.

We can also customize related analogues and modified peptides including HPLC, MS, 1H-NMR, MS, HPLC, IR, UV, COA, MSDS.

This product is intended for laboratory use only!

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E-mail: anna@molcoo.com

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