文章标题：miR-34a-5p blocks cervical cancer growth and migration by downregulating CDC25A.
期刊：Journal of BUON
作者列表：Jiang T, Cheng H
发表时间：2021-9-1
影响因子：2.533
DOI:PMID:34761581
主要研究成果Abstract
Purpose
To define mechanisms underlying the regulation of CDC25A by miR-34a-5p in cervical cancer (CC).
Methods
Quantifications of miR-34a-5p and CDC25A levels were performed in 68 CC tissues and matched controls. CC cell lines HeLa and SiHa were transfected to monitor their biological behavior changes.
Results
Low-expressed miR-34a-5p and up-regulated CDC25A were noted in CC. Either overexpression of miR-34a-5p or inhibition of CDC25A blocked the growth and migration of HeLa and SiHa cells, resulting in an increase in apoptosis (p<0.05), while inhibition of miR-34a-5p or overexpression of CDC25A promoted tumor growth. Dual-luciferase reporter (DLR) assay and cell transfection identified the targeting relationship between miR-34a-5p and CDC25A, and correlation analysis revealed a negative association between them in CC. Co-transfection assays showed that overexpression of CDC25A reversed the inhibition of miR-34a-5p on CC growth and migration.