基本信息
产品名称:Histone H4 Mouse mAb
规格或纯度:ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
应用:WB, IF/ICC
特异性:Histone H4
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原:Recombinant full length protein corresponding to Human H4C1
阳性对照:WB: Hela, A431, MCF-7, NIH/3T3, C6, Jurkat, K562, A549, LNCaP whole cell lysates ICC/IF: HeLa cells
偶联:Unconjugated
产品属性
克隆类型:单克隆抗体
克隆号:CD01/2H7
Format:Whole IgG
亚型:IgG2b
轻链亚型:kappa
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:PBS, pH 7.2
防腐剂:No
浓度:1.0 mg/mL
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:在 +4°C 下短期储存(1-2 周)。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:小鼠(Mouse)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆号:CD01/2H7
偶联:Unconjugated
图片

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Western Blot: Histone H4 Mouse mAb (Ab156574)
Histone H4 Mouse mAb (Ab156574) - Western Blot
All lanes: Histone H4 Antibody (Ab156574) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 11.4 kDa
Observed band size: 13.6 kDa
Exposure time: 2.4 s

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Western Blot: Histone H4 Mouse mAb (Ab156574)
Histone H4 Mouse mAb (Ab156574) - Western Blot
All lanes: Histone H4 Antibody (Ab156574) at 1/10000 dilution
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 11.4 kDa
Observed band size: 13.5 kDa
Exposure time: 60.0 s

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ICC/IF: Histone H4 Mouse mAb (Ab156574)
Histone H4 Mouse mAb (Ab156574) - ICC/IF
IF analysis of Histone H4 (Red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Histone H4 Mouse mAb (Ab156574) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab156574.html



关键字: H4 成簇组蛋白 1 ; Anti-Histone H1 抗体 ; 组蛋白H4小鼠mAb;dJ160A22.1 antibody
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