基本信息
产品名称:Hck Mouse mAb
规格或纯度:ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL
应用:WB, IF/ICC
特异性:Hck
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原:Recombinant human HCK expressed in E.coli (AA 1-526).
阳性对照:WB: THP-1 and Hep G2 cell lysates. ICC/IF: Hep G2 cells.
偶联:Unconjugated
产品属性
克隆类型:单克隆抗体
克隆号:1508CT602.13.1
Format:Whole IgG
亚型:Mouse IgG1
轻链亚型:kappa
SDS-PAGE:150 kDa
纯化方法:Protein G purified
物理外观:Liquid
储存缓冲液:10mM PBS, 0.09% Sodium azide, pH7.4
防腐剂:0.09% Sodium azide
浓度:0.5 mg/mL
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:小鼠(Mouse)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆号:1508CT602.13.1
偶联:Unconjugated
图片

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Western Blot: Hck Mouse mAb (Ab106982)
Hck Mouse mAb (Ab106982) - Western Blot
All lanes: Hck Mouse mAb (Ab106982) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
Exposure time: 10.0 s

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Western Blot: Hck Mouse mAb (Ab106982)
Hck Mouse mAb (Ab106982) - Western Blot
All lanes: Hck Mouse mAb (Ab106982) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
Exposure time: 60.0 s

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IF/ICC: Hck Mouse mAb (Ab106982)
Hck Mouse mAb (Ab106982) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hep G2 (human liver hepatocellular carcinoma cell line) cells labeling HCK with Hck Mouse mAb (Ab106982) at 1/25 dilution,
followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab106982.html



关键字: 造血细胞激酶(HCK)抗体 ; Hck抗体 ; 造血细胞激酶(HCK)抗体 ; Hck抗体
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