Rabbit Polyclonal MOB1B Antibody

The MOB1B antibody is a crucial tool for studying the MOB1B protein, a member of the MOB (Mps One Binder) superfamily involved in regulating cell cycle progression, apoptosis, and the Hippo signaling pathway. MOB1B, along with its homolog MOB1A, acts as a conserved component of the Hippo pathway, which controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis. MOB1B interacts with MST1/2 kinases and LATS1/2 kinases, facilitating their phosphorylation and activation. This cascade ultimately leads to the inactivation of YAP/TAZ transcriptional co-activators, critical for cell growth and survival. Dysregulation of MOB1B is implicated in cancers, as altered Hippo signaling often correlates with tumorigenesis and metastasis.

MOB1B antibodies are widely used in Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous MOB1B expression, localization, and protein interactions. They help elucidate MOB1B's role in maintaining genomic stability, mitotic exit, and its crosstalk with other pathways like Wnt or Notch. Researchers also employ these antibodies to investigate MOB1B's differential expression in cancer tissues versus normal tissues, its prognostic significance, and responses to targeted therapies. Specificity is critical, as MOB1B shares high sequence similarity with MOB1A, requiring rigorous validation via knockout/knockdown controls. Such antibodies are indispensable for understanding MOB1B's multifaceted roles in development, homeostasis, and disease.