GAPDHS抗体,Rabbit Polyclonal GAPDHS Antibody
  • GAPDHS抗体,Rabbit Polyclonal GAPDHS Antibody
  • GAPDHS抗体,Rabbit Polyclonal GAPDHS Antibody
  • GAPDHS抗体,Rabbit Polyclonal GAPDHS Antibody

GAPDHS抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-09
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产品详情

中文名称:GAPDHS抗体英文名称:Rabbit Polyclonal GAPDHS Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 283 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: GAPDHS
2025-05-09 GAPDHS抗体 Rabbit Polyclonal GAPDHS Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 283 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/5000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesGAPD2; GAPDS; HSD-35; GAPDH-2; HEL-S-278
WB Predicted band size45 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthetic peptide of human GAPDHS
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: A549 cell lysate, Primary antibody: P04645(GAPDHS Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes    


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       The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P04645(GAPDHS Antibody) at dilution 1/50. (Original magnification: ×200)    


           

参考文献

以下是关于GAPDHS抗体的3篇参考文献的简要信息:

1. **文献名称**:Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility

**作者**:Miki, K. et al.

**摘要**:该研究通过基因敲除小鼠模型,发现GAPDHS缺失导致精子能量代谢障碍和运动能力丧失,最终引发雄性不育。研究中使用GAPDHS抗体验证了蛋白在精子中的特异性表达及敲除后蛋白的缺失。

2. **文献名称**:Mammalian sperm energy metabolism: oxygenation and phosphorylation of glyceraldehyde 3-phosphate dehydrogenase-S (GAPDHS)

**作者**:Nakamura, N. et al.

**摘要**:探讨GAPDHS在精子糖酵解中的调控机制,通过抗体进行免疫荧光定位,发现该酶集中分布于精子尾部线粒体鞘,提示其直接参与鞭毛运动的能量供应。

3. **文献名称**:Cloning and expression of a novel tissue-specific isoform of glyceraldehyde-3-phosphate dehydrogenase from mouse testis

**作者**:Welch, J.E. et al.

**摘要**:首次报道小鼠GAPDHS基因的克隆及睾丸特异性表达,通过制备特异性抗体证实该蛋白仅在精子发生晚期表达,为后续功能研究奠定基础。

*注:如需完整文献信息,建议通过PubMed或Sci-Hub输入标题查询。*

       

背景信息

The GAPDHS antibody targets a unique isoform of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), specifically expressed in spermatogenic cells during mammalian spermatogenesis. Unlike the ubiquitously expressed GAPDH involved in glycolysis, GAPDHS (Glyceraldehyde-3-phosphate dehydrogenase, spermatogenic) is testis-specific and plays a critical role in sperm metabolism, motility, and fertilization. Its expression is tightly regulated during spermatogenesis, peaking in late-stage germ cells and mature spermatozoa.

GAPDHS antibodies are essential tools for studying male fertility, testicular function, and sperm development. They enable the detection and localization of GAPDHS protein in testicular tissues or sperm samples using techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Research using these antibodies has highlighted GAPDHS's role in maintaining energy homeostasis in sperm, particularly in the flagellum, where it supports ATP production for motility.

Dysregulation or mutations in GAPDHS are linked to asthenozoospermia (reduced sperm motility) and male infertility, making it a potential biomarker for diagnostic or therapeutic applications. Additionally, GAPDHS antibodies aid in exploring metabolic adaptations in sperm and evaluating environmental or genetic factors affecting reproductive health. Their specificity ensures minimal cross-reactivity with somatic GAPDH, enhancing reliability in reproductive biology studies.

       
关键字: GAPDHS抗体;GAPDHS;GAPDHS Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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