NCOA6 (Nuclear Receptor Coactivator 6), also known as ASC-2 or AIB3. is a transcriptional coactivator that interacts with nuclear receptors (e.g., estrogen, thyroid, and retinoid receptors) and other transcription factors to regulate gene expression. It plays a role in diverse cellular processes, including cell proliferation, differentiation, metabolism, and DNA repair. Structurally, it contains multiple activation domains and LXXLL motifs critical for binding nuclear receptors. Dysregulation of NCOA6 has been implicated in cancers (e.g., breast, liver) and metabolic disorders, making it a subject of interest in molecular research.
NCOA6 antibodies are essential tools for studying its expression, localization, and function. These antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specificity is confirmed via knockout controls or siRNA-mediated depletion. Researchers use these antibodies to explore NCOA6's role in transcriptional networks, hormone signaling, and disease mechanisms. Commercial suppliers often provide detailed validation data, including reactivity across species (human, mouse, rat) and recommended experimental conditions. Reliable NCOA6 antibodies are critical for elucidating its dual roles as an oncogenic collaborator or tumor suppressor, depending on cellular context.