ALOX15 (Arachidonate 15-lipoxygenase) is an enzyme belonging to the lipoxygenase family, which catalyzes the oxidation of polyunsaturated fatty acids, particularly converting arachidonic acid to 15-hydroperoxyeicosatetraenoic acid (15-HPETE). This enzyme plays a dual role in inflammation and homeostasis, implicated in both pro-inflammatory and pro-resolving processes. It is expressed in immune cells, such as macrophages and eosinophils, and is involved in cellular signaling, immune responses, and lipid metabolism. Dysregulation of ALOX15 has been linked to various pathologies, including atherosclerosis, neurodegenerative diseases (e.g., Alzheimer’s), and cancer, where it may promote tumor progression or suppression depending on context.
ALOX15 antibodies are essential tools for detecting and quantifying the enzyme’s expression in tissues or cells. They are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to study its localization, regulation, and function. Recent research highlights ALOX15’s role in ferroptosis, a form of iron-dependent cell death, making it a potential therapeutic target. Antibodies specific to ALOX15 help elucidate its involvement in disease mechanisms and evaluate its suitability as a biomarker or drug target. Commercial antibodies often target epitopes in conserved regions to ensure cross-species reactivity. However, variability in antibody specificity requires validation via knockout controls to avoid off-target effects. Ongoing studies aim to clarify ALOX15’s context-dependent roles, driving demand for reliable antibodies in both basic and translational research.