The CRTC1 (CREB-regulated transcription coactivator 1) antibody is a tool used to study the CRTC1 protein, a key regulator of cellular transcription. CRTC1. part of the CRTC family (CRTC1-3), acts as a coactivator for CREB (cAMP response element-binding protein), mediating gene expression in response to cAMP signaling and other pathways. It plays critical roles in energy homeostasis, circadian rhythms, neuronal plasticity, and stress responses. Dysregulation of CRTC1 is linked to metabolic disorders, cancer, and neurodegenerative diseases.
CRTC1 antibodies are typically produced using recombinant protein fragments or peptides as immunogens, often validated via Western blot, immunohistochemistry, or immunofluorescence. These antibodies help detect CRTC1’s expression, localization (e.g., cytoplasmic retention under inactive states vs. nuclear translocation upon activation), and post-translational modifications (e.g., phosphorylation regulating its activity). Studies using CRTC1 antibodies have elucidated its interaction with CREB, involvement in gluconeogenesis, and role in memory formation. In cancer research, CRTC1 antibodies aid in investigating its oncogenic or tumor-suppressive functions, depending on context. Challenges include ensuring specificity due to homology among CRTC family members. Proper validation using knockout controls is essential. Overall, CRTC1 antibodies are vital for exploring CREB/CRTC1 signaling and its therapeutic implications.