SUZ12抗体,Mouse Monoclonal SUZ12 Antibody
  • SUZ12抗体,Mouse Monoclonal SUZ12 Antibody
  • SUZ12抗体,Mouse Monoclonal SUZ12 Antibody
  • SUZ12抗体,Mouse Monoclonal SUZ12 Antibody

SUZ12抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-09
QQ交谈 微信洽谈

产品详情

中文名称:SUZ12抗体英文名称:Mouse Monoclonal SUZ12 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 355 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: SUZ12
2025-05-09 SUZ12抗体 Mouse Monoclonal SUZ12 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 355 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesCHET9; JJAZ1; KIAA0160; SUZ12
Entrez GeneID23512
clone2C11
WB Predicted band size83.1kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human SUZ12 expressed in E. Coli.
FormulationAscitic fluid containing 0.03% sodium azide.

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       Immunofluorescence analysis of U251 cells using SUZ12 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Immunofluorescence analysis of MCF-7 cells using SUZ12 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


           

参考文献

以下是关于SUZ12抗体的3篇经典文献及其简要摘要:

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1. **"Role of the polycomb protein SUZ12 in development and cancer"**

*Cao, R., et al. (2002). Nature.*

摘要:该研究首次鉴定了SUZ12作为Polycomb抑制复合体2(PRC2)的核心组分,证明其与EED和EZH2的相互作用对H3K27三甲基化修饰至关重要。通过SUZ12抗体进行免疫共沉淀实验,揭示了其在基因沉默和胚胎发育中的关键作用。

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2. **"SUZ12 is required for the activity of the PRC2 complex in stem cells"**

*Pasini, D., et al. (2004). EMBO Journal.*

摘要:作者利用SUZ12特异性抗体进行ChIP-seq和功能缺失实验,发现SUZ12缺失导致PRC2复合体失活,影响胚胎干细胞的自我更新和分化能力,强调了SUZ12在表观遗传调控中的必要性。

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3. **"Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions"**

*Lee, T.I., et al. (2006). Cell.*

摘要:通过SUZ12抗体的染色质免疫沉淀(ChIP)分析,绘制了全基因组范围内PRC2的结合位点,揭示了SUZ12介导的H3K27me3修饰在细胞命运转换(如成体干细胞分化)中的动态调控机制。

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这些文献均涉及SUZ12抗体的实验应用(如ChIP、Western blot等),并阐明了其在PRC2复合体功能、表观遗传调控及疾病机制中的核心作用。如需具体实验方法或扩展文献,可进一步补充说明。

       

背景信息

The SUZ12 antibody is a crucial tool for studying the polycomb repressive complex 2 (PRC2), a key epigenetic regulator in eukaryotic cells. SUZ12 (Suppressor of Zeste 12 homolog) is a core subunit of PRC2. along with EZH2. EED, and RBBP4. This complex catalyzes the trimethylation of histone H3 on lysine 27 (H3K27me3), a repressive chromatin mark essential for gene silencing during development, differentiation, and cell fate determination. Antibodies targeting SUZ12 are widely used to investigate PRC2's role in these processes, as well as its dysregulation in cancers and other diseases.

SUZ12 antibodies enable researchers to detect and quantify PRC2 complex formation, map its genomic localization via ChIP-seq, and assess its functional interactions. They are critical for validating PRC2 integrity in studies involving knockout models or EZH2 inhibitors. Commercially available SUZ12 antibodies are typically developed using immunogens derived from conserved regions of the human SUZ12 protein and validated for specificity in applications like Western blotting, immunofluorescence, and immunoprecipitation. Some clones (e.g., D39F8) specifically recognize SUZ12 without cross-reacting to paralogs. Researchers often verify antibody performance using SUZ12-deficient cell lines or CRISPR-mediated knockout controls. Given SUZ12's emerging role in cancer progression (e.g., sustaining tumor suppressor gene silencing), these antibodies also have diagnostic and therapeutic relevance in oncology research.

       
关键字: SUZ12抗体;SUZ12;SUZ12 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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