ABCG2抗体,Mouse Monoclonal ABCG2 Antibody
  • ABCG2抗体,Mouse Monoclonal ABCG2 Antibody
  • ABCG2抗体,Mouse Monoclonal ABCG2 Antibody
  • ABCG2抗体,Mouse Monoclonal ABCG2 Antibody

ABCG2抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-09
QQ交谈 微信洽谈

产品详情

中文名称:ABCG2抗体英文名称:Mouse Monoclonal ABCG2 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 408 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: ABCG2
2025-05-09 ABCG2抗体 Mouse Monoclonal ABCG2 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 408 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/100 - 1/500 Human,Mouse,Rat
ICC1/50 - 1/500 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesMRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; CDw338; EST157481; MGC102821
Entrez GeneID9429
clone3G8
WB Predicted band size72kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey
ImmunogenPurified recombinant fragment of human ABCG2 expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    


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       Western blot analysis using ABCG2 mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.    


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       Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple).    


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       Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Mouse muscle using ABCG2 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rat muscle using ABCG2 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rabbit muscle using ABCG2 mouse mAb with DAB staining.    


           

参考文献

以下是3-4条关于ABCG2抗体的参考文献及其摘要内容:

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1. **文献名称**:*Multidrug resistance mediated by the breast cancer resistance protein (ABCG2)*

**作者**:Doyle, L.A., & Ross, D.D.

**摘要**:该研究首次克隆并表征了ABCG2(BCRP)基因,揭示了其作为ATP结合盒转运蛋白在肿瘤多药耐药中的作用,并开发了特异性抗体用于检测其在癌细胞中的过表达。

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2. **文献名称**:*Subcellular localization and distribution of the breast cancer resistance protein transporter in normal human tissues*

**作者**:Maliepaard, M., et al.

**摘要**:通过免疫组化分析,研究利用ABCG2特异性抗体揭示了该蛋白在胎盘、肝脏、小肠等正常组织中的分布特征,为理解其生理功能及药物外排机制提供依据。

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3. **文献名称**:*Comparison of anti-ABCG2 monoclonal antibodies for specificity and sensitivity in immunohistochemistry*

**作者**:Mao, Q., & Minderman, H.

**摘要**:研究系统评估了多种商业ABCG2抗体的特异性和灵敏度,发现部分抗体存在交叉反应性,强调选择高特异性抗体对临床样本分析的重要性。

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4. **文献名称**:*Breast cancer resistance protein (BCRP/ABCG2): its role in multidrug resistance and regulation of gene expression*

**作者**:Nakanishi, T., & Ross, D.D.

**摘要**:综述总结了ABCG2在肿瘤耐药中的分子机制,并讨论了抗体在检测其表达水平及功能研究中的应用,强调其在个体化治疗中的潜在价值。

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以上文献涵盖了ABCG2的功能研究、抗体开发及临床应用,可作为相关领域的基础参考。

       

背景信息

ABCG2 (ATP-binding cassette subfamily G member 2), also known as breast cancer resistance protein (BCRP), is a transmembrane transporter protein belonging to the ATP-binding cassette (ABC) superfamily. It plays a critical role in cellular detoxification by effluxing a wide range of endogenous and exogenous substrates, including chemotherapeutic agents, toxins, and metabolic byproducts. ABCG2 is expressed in various tissues, such as the placenta, blood-brain barrier, liver, and intestines, where it contributes to tissue protection and drug pharmacokinetics. Its overexpression in cancer cells is linked to multidrug resistance (MDR), a major challenge in oncology, as it reduces intracellular drug accumulation and therapeutic efficacy.

ABCG2 antibodies are essential tools for studying the protein's expression, localization, and functional roles. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and flow cytometry, aiding in research on drug resistance mechanisms, stem cell biology (e.g., side population identification), and barrier tissue functions. Monoclonal and polyclonal antibodies targeting specific epitopes of ABCG2 are validated for specificity and sensitivity in diverse experimental models. Recent advances in therapeutic strategies targeting ABCG2. such as inhibitors to overcome MDR or nanoparticle-based drug delivery systems, further underscore the importance of reliable antibodies in both basic research and clinical applications.

       
关键字: ABCG2抗体;ABCG2;ABCG2 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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