OTX2抗体

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

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     Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.    

  
  

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     Immunofluorescence analysis of HepG2 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Flow cytometric analysis of HepG2 cells using OTX2 mouse mAb (green) and negative control (purple).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human prostate tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Mouse testis using OTX2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat brain using OTX2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat kidney using OTX2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rabbit kidney using OTX2 mouse mAb with DAB staining.    

  
  

The OTX2 antibody is a crucial tool in developmental biology and oncology research, targeting the Orthodenticle Homeobox 2 (OTX2) protein, a transcription factor encoded by the OTX2 gene. OTX2 plays a pivotal role in embryonic development, particularly in the formation of the forebrain, eyes, and pituitary gland. It regulates gene expression critical for neuroectoderm patterning, photoreceptor differentiation, and craniofacial development. Dysregulation of OTX2 has been implicated in various diseases, including medulloblastoma, retinoblastoma, and retinal degeneration, making it a biomarker of interest in cancer and ocular disorders.

Researchers use OTX2 antibodies to detect and localize the protein in tissues or cells via techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence. These applications help elucidate OTX2's expression patterns during development and disease progression. In cancer, OTX2 overexpression is linked to tumor aggressiveness and poor prognosis, driving studies on its role in oncogenesis and potential therapeutic targeting. Additionally, OTX2 antibodies aid in studying congenital disorders, such as microphthalmia and pituitary dysfunction, where OTX2 mutations disrupt normal development. Available as monoclonal or polyclonal variants, these antibodies are validated for specificity and sensitivity across species, including humans and mice, supporting diverse experimental models. Overall, OTX2 antibodies are indispensable for advancing understanding of developmental mechanisms and disease pathways.