BLNK抗体,Mouse Monoclonal BLNK Antibody
  • BLNK抗体,Mouse Monoclonal BLNK Antibody
  • BLNK抗体,Mouse Monoclonal BLNK Antibody
  • BLNK抗体,Mouse Monoclonal BLNK Antibody

BLNK抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-09
QQ交谈 微信洽谈

产品详情

中文名称:BLNK抗体英文名称:Mouse Monoclonal BLNK Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 419 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: BLNK
2025-05-09 BLNK抗体 Mouse Monoclonal BLNK Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 419 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat,,Monkey
IF咨询技术 Human,Mouse,Rat,,Monkey
IHC1/200 - 1/1000 Human,Mouse,Rat,,Monkey
ICC1/200 - 1/1000 Human,Mouse,Rat,,Monkey
FCM1/200 - 1/400 Human,Mouse,Rat,,Monkey
Elisa1/10000 Human,Mouse,Rat,,Monkey
   

产品详情

AliasesAGM4; BASH; LY57; SLP65; BLNK-S; SLP-65; MGC111051
Entrez GeneID29760
clone5G9
WB Predicted band size68kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,,Monkey
ImmunogenPurified recombinant fragment of human BLNK expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    


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       Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.    


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       Western blot analysis using BLNK mouse mAb against CHO3D10(1),COS7(2),L1210(3),C6(4),NIH/3T3(5),PC-12(6),Mouse liver(7) cell lysate.    


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       Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining.    


           

参考文献

以下是关于BLNK抗体的3-4篇参考文献示例(文献为模拟,供参考):

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1. **文献名称**: *BLNK: A Central Scaffold Protein in B Cell Receptor Signaling*

**作者**: Fu, C., et al.

**摘要**: 本研究通过BLNK特异性抗体,揭示了BLNK在B细胞受体(BCR)信号中的支架作用。实验表明,BLNK通过招募PLCγ2和Vav等信号分子,调控钙离子流动和细胞骨架重组,是B细胞活化的关键中介。

2. **文献名称**: *Impaired B Cell Development in BLNK-Deficient Mice*

**作者**: Ishiai, M., et al.

**摘要**: 利用BLNK抗体检测基因敲除小鼠模型,发现BLNK缺失导致前B细胞向成熟B细胞分化停滞,证实BLNK在B细胞发育中不可或缺,并影响免疫球蛋白重链的编辑。

3. **文献名称**: *BLNK Mediates Syk-Dependent Signaling Pathways in B Cells*

**作者**: Kurosaki, T., et al.

**摘要**: 通过共免疫沉淀实验(使用BLNK抗体),阐明BLNK与Syk激酶的结合促进下游MAPK和NF-κB通路的激活,为B细胞增殖和存活提供分子机制依据。

4. **文献名称**: *BLNK Expression Correlates with Autoimmunity Severity*

**作者**: Wang, L., et al.

**摘要**: 在系统性红斑狼疮(SLE)患者中,采用BLNK抗体进行免疫组化分析,发现BLNK在过度活跃的B细胞中高表达,提示其可作为疾病生物标志物或治疗靶点。

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**注**:以上文献为示例性内容,实际引用时需核实真实文献来源及准确性。建议通过PubMed或Google Scholar以关键词“BLNK antibody”或“BLNK signaling”检索最新研究。

       

背景信息

**Background of BLNK Antibody**

BLNK (B-cell linker protein), also known as SLP-65 or BASH, is a critical adaptor protein in B-cell receptor (BCR) signaling. It is encoded by the *BLNK* gene and primarily expressed in B lymphocytes. BLNK plays a pivotal role in bridging activated BCR-associated kinases (e.g., Syk) to downstream signaling pathways, including PLCγ activation, calcium mobilization, and MAPK/NF-κB regulation. This interaction is essential for B-cell development, differentiation, and immune response coordination.

BLNK deficiency disrupts BCR signal transduction, leading to impaired B-cell maturation and antibody production. Mutations in *BLNK* are linked to agammaglobulinemia, a primary immunodeficiency characterized by low antibody levels and recurrent infections. Additionally, aberrant BLNK expression has been observed in B-cell malignancies, suggesting its role in oncogenic signaling.

BLNK-specific antibodies are vital tools for studying B-cell biology and pathology. They enable detection of BLNK expression, phosphorylation status, and interactions in immune assays (e.g., Western blot, immunoprecipitation). Research applications include investigating immune disorders, leukemia mechanisms, and therapeutic targets for modulating B-cell activity. These antibodies also aid in diagnosing genetic defects affecting BCR signaling pathways.

In summary, BLNK antibodies provide insights into B-cell function, disease mechanisms, and potential immunotherapies, highlighting their importance in both basic and clinical immunology.

       
关键字: BLNK抗体;BLNK;BLNK Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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