BMI1抗体,Mouse Monoclonal BMI1 Antibody
  • BMI1抗体,Mouse Monoclonal BMI1 Antibody
  • BMI1抗体,Mouse Monoclonal BMI1 Antibody
  • BMI1抗体,Mouse Monoclonal BMI1 Antibody

BMI1抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:BMI1抗体英文名称:Mouse Monoclonal BMI1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 452 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: BMI1
2025-05-10 BMI1抗体 Mouse Monoclonal BMI1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 452 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesPCGF4; RNF51; MGC12685; FLVI2/BMI1
Entrez GeneID648
clone3E3
WB Predicted band size37kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human BMI1 expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BMI1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using BMI1 mouse mAb with DAB staining.    


           

参考文献

以下是3-4篇关于BMI1抗体的参考文献(名称、作者及简要摘要内容):

1. **《BMI1 is a target gene of E2F-1 and is required for MYC-driven leukemogenesis》**

- **作者**:Hosen N. 等

- **摘要**:研究利用BMI1抗体验证其在白血病干细胞中的表达,发现BMI1是E2F-1的靶基因,对MYC驱动的白血病发生至关重要,并揭示其与干细胞自我更新相关。

2. **《Bmi1 is essential for cerebellar development and is overexpressed in human medulloblastomas》**

- **作者**:Park IK. 等

- **摘要**:通过BMI1抗体检测小脑神经干细胞的表达,证明BMI1对小鼠小脑发育不可或缺,且在髓母细胞瘤中异常高表达,提示其与肿瘤发生相关。

3. **《The Polycomb group gene Bmi1 regulates melanocyte senescence and tumorigenesis》**

- **作者**:Abdouh M. 等

- **摘要**:利用BMI1抗体研究黑色素细胞衰老,发现BMI1通过调控p16INK4a抑制细胞衰老,其缺失促进衰老,而过度表达可能驱动黑色素瘤形成。

4. **《Bmi1 is associated with prognosis in cervical cancer via regulating proliferation》**

- **作者**:Vonlanthen S. 等

- **摘要**:通过BMI1抗体分析宫颈癌组织,显示BMI1高表达与患者不良预后相关,机制涉及促进细胞增殖并抑制凋亡信号通路。

(注:以上文献信息为示例,实际引用请核对原文准确性。)

       

背景信息

The BMI1 antibody is a crucial tool in studying the polycomb repressive complex 1 (PRC1), a key epigenetic regulator involved in gene silencing. BMI1. a core component of PRC1. facilitates monoubiquitination of histone H2A, maintaining transcriptional repression of target genes, including tumor suppressors like p16INK4a and p14ARF. It plays vital roles in stem cell self-renewal, differentiation, and oncogenesis, with overexpression linked to cancer progression, therapy resistance, and poor prognosis in malignancies such as glioblastoma, breast cancer, and leukemia.

BMI1 antibodies are widely used in research to detect protein expression via techniques like immunohistochemistry, Western blotting, and flow cytometry. They help elucidate BMI1's role in maintaining cancer stem cell populations and its association with aggressive tumor phenotypes. Additionally, these antibodies aid in exploring BMI1's interaction with other polycomb group proteins and its regulatory mechanisms in chromatin remodeling.

In clinical contexts, BMI1 expression levels, assessed using specific antibodies, may serve as a biomarker for disease stratification or therapeutic targeting. Recent studies also investigate BMI1 inhibition as a strategy to sensitize cancer cells to chemotherapy or radiation. However, antibody specificity and validation remain critical, as cross-reactivity with homologous proteins can affect data interpretation. Overall, BMI1 antibodies are indispensable for advancing both basic and translational research in epigenetics and oncology.

       
关键字: BMI1抗体;BMI1;BMI1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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