YAP1抗体,Mouse Monoclonal YAP1 Antibody
  • YAP1抗体,Mouse Monoclonal YAP1 Antibody
  • YAP1抗体,Mouse Monoclonal YAP1 Antibody
  • YAP1抗体,Mouse Monoclonal YAP1 Antibody

YAP1抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:YAP1抗体英文名称:Mouse Monoclonal YAP1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 479 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: YAP1
2025-05-10 YAP1抗体 Mouse Monoclonal YAP1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 479 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat,Rabbit
IF咨询技术 Human,Mouse,Rat,Rabbit
IHC1/100 - 1/500 Human,Mouse,Rat,Rabbit
ICC技术咨询 Human,Mouse,Rat,Rabbit
FCM1/200 - 1/400 Human,Mouse,Rat,Rabbit
Elisa1/10000 Human,Mouse,Rat,Rabbit
   

产品详情

AliasesYAP; YKI; YAP2; YAP65
Entrez GeneID10413
clone1A12
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Monkey,Rabbit
ImmunogenPurified recombinant fragment of human YAP1 expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Western blot analysis using YAP1 mouse mAb against Hela (1), C6 (2) and Cos7 (3) cell lysate.    


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       Western blot analysis using YAP1 mouse mAb against mouse liver(1)PC-12 (2) NIH/3T3(3)NRK (4) C2C12(5)C6(6)F9(7) COS-7(8)MCF-7(9) cell lysate.    


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       Flow cytometric analysis of Hela cells using YAP1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using YAP1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using YAP1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Mouse kidney,Rat kidney,Rabbit kidney using YAP1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rat kidney using YAP1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rabbit kidney using YAP1 mouse mAb with DAB staining.    


           

参考文献

以下是关于YAP1抗体的3篇参考文献示例(文献信息为虚构,仅供示例参考):

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1. **"YAP1 overexpression correlates with poor prognosis in hepatocellular carcinoma"**

*Authors: Li, X.; Wang, Y.; Chen, J.*

**摘要**:本研究通过免疫组化(IHC)和Western blot分析,发现YAP1蛋白在肝细胞癌(HCC)组织中显著高表达,并与患者生存率降低相关。研究使用特异性YAP1抗体验证其作为潜在预后标志物的价值。

2. **"YAP1 phosphorylation regulates its nuclear localization in breast cancer cells"**

*Authors: Johnson, R.; Smith, K.; Davis, M.*

**摘要**:文章揭示了Hippo通路激酶LATS1介导的YAP1磷酸化调控其核质穿梭的机制。通过YAP1抗体进行免疫荧光染色,证实磷酸化缺陷突变体YAP1在细胞核内持续积累,促进乳腺癌细胞增殖。

3. **"YAP1-TEAD interaction drives chemoresistance in colorectal cancer"**

*Authors: Gupta, S.; Patel, T.; Nguyen, H.*

**摘要**:研究利用YAP1抗体进行染色质免疫沉淀(ChIP),证明YAP1与TEAD转录因子结合后激活抗凋亡基因表达,导致结直肠癌细胞对5-氟尿嘧啶耐药。抑制YAP1可逆转这一效应。

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如需真实文献,建议在PubMed或Google Scholar中搜索关键词“YAP1 antibody”、“YAP1 cancer”或“YAP1 Hippo pathway”获取。

       

背景信息

YAP1 (Yes-associated protein 1) is a transcriptional co-activator that plays a pivotal role in the Hippo signaling pathway, which regulates organ size, cell proliferation, apoptosis, and stem cell maintenance. It functions by shuttling between the cytoplasm and nucleus: when the Hippo pathway is active, YAP1 undergoes phosphorylation and cytoplasmic retention; when inactive, unphosphorylated YAP1 translocates to the nucleus, where it interacts with transcription factors (e.g., TEADs) to drive pro-growth and anti-apoptotic gene expression. Dysregulation of YAP1 is implicated in cancers, fibrosis, and developmental disorders due to its oncogenic potential and role in tissue regeneration.

YAP1 antibodies are essential tools for detecting YAP1 expression, localization, and activation status in research. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study Hippo pathway dynamics, cancer progression, and therapeutic targeting. Specific antibodies can distinguish between phosphorylated (inactive) and non-phosphorylated (active) YAP1 isoforms, aiding in mechanistic studies. Commercial YAP1 antibodies are often validated for cross-reactivity in human, mouse, and rat samples. Recent studies also explore YAP1's role in mechanotransduction, immune modulation, and regenerative medicine, further driving demand for reliable antibodies to dissect its multifaceted biological functions.

       
关键字: YAP1抗体;YAP1;YAP1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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