The NCK1 (non-catalytic region of tyrosine kinase adaptor protein 1) antibody is a tool used to study the NCK1 protein, a key intracellular adaptor involved in signal transduction pathways. NCK1 contains one SH2 domain and three SH3 domains, enabling it to bind phosphorylated tyrosine residues and proline-rich motifs, respectively. It acts downstream of receptor tyrosine kinases (RTKs) and integrins, mediating interactions between signaling molecules to regulate processes like cytoskeletal reorganization, cell migration, and proliferation. NCK1 antibodies are widely employed in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect NCK1 expression, localization, and protein-protein interactions in various cellular contexts. Research has linked NCK1 to diseases including cancer, inflammation, and developmental disorders, highlighting its role in pathological signaling. Commercially available NCK1 antibodies are often raised against specific epitopes, with validation data confirming specificity across human, mouse, and rat samples. Studies using these antibodies have revealed NCK1's involvement in pathways like MAPK/ERK and its crosstalk with oncogenic signals, making it a potential therapeutic target. Proper antibody selection and validation are critical to avoid cross-reactivity with its homolog NCK2. which shares structural similarities.