MAP1LC3B抗体,Mouse Monoclonal MAP1LC3B Antibody
  • MAP1LC3B抗体,Mouse Monoclonal MAP1LC3B Antibody
  • MAP1LC3B抗体,Mouse Monoclonal MAP1LC3B Antibody
  • MAP1LC3B抗体,Mouse Monoclonal MAP1LC3B Antibody

MAP1LC3B抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-10
QQ交谈 微信洽谈

产品详情

中文名称:MAP1LC3B抗体英文名称:Mouse Monoclonal MAP1LC3B Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 510 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: MAP1LC3B
2025-05-10 MAP1LC3B抗体 Mouse Monoclonal MAP1LC3B Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 510 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC1/50-1/100 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesLC3B; ATG8F; MAP1A/1BLC3
Entrez GeneID81631
clone5H12
WB Predicted band size14.6kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Monkey
ImmunogenPurified recombinant fragment of human MAP1LC3B expressed in E. Coli.  
FormulationAscitic fluid containing 0.03% sodium azide.

+ +

  •  

     

       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


  •  

     

       Immunofluorescence analysis of Hela cells using MAP1LC3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    


  •  

     

       Flow cytometric analysis of HeLa cells using MAP1LC3B mouse mAb (green) and negative control (red).    


  •  

     

       Flow cytometric analysis of C6 cells using MAP1LC3B mouse mAb (green) and negative control (red).    


  •  

     

       Flow cytometric analysis of  NIH/3T3 cells using MAP1LC3B mouse mAb (green) and negative control (red).    


  •  

     

       Immunohistochemical analysis of paraffin-embedded Mouse cerebellum using MAP1LC3B mouse mAb with DAB staining.    


  •  

     

       Immunohistochemical analysis of paraffin-embedded Rat cerebellum using MAP1LC3B mouse mAb with DAB staining.    


           

参考文献

以下是关于MAP1LC3B抗体的3篇代表性文献的简要总结:

1. **文献名称**:*LC3. a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing*

**作者**:Kabeya, Y. et al.

**摘要**:该研究首次鉴定并描述了哺乳动物LC3蛋白(包括LC3B)的功能,阐明了其在自噬体形成中的关键作用。作者开发了特异性识别LC3B的抗体,验证了其在免疫印迹(Western blot)和免疫荧光中的应用,证明了LC3B-II(脂化形式)作为自噬体标志物的可靠性。

2. **文献名称**:*Guidelines for the use and interpretation of assays for monitoring autophagy*

**作者**:Klionsky, D.J. et al.

**摘要**:这篇权威指南汇总了自噬研究的标准化方法,其中专门讨论了LC3B抗体的应用场景(如Western blot检测LC3B-I/II转化、免疫荧光定位自噬体)。文献强调了不同抗体(如抗N端或C端抗体)的适用性差异,并提醒注意假阳性结果的潜在问题。

3. **文献名称**:*Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3*

**作者**:Mizushima, N. et al.

**摘要**:研究通过构建串联荧光标记的LC3B(mRFP-GFP-LC3)探针,开发了动态监测自噬流的方法。文中对比了多种商业LC3B抗体的特异性,验证了其在流式细胞术和共聚焦显微镜中的有效性,为抗体选择提供了实验依据。

4. **文献名称**:*LC3 and Autophagy*

**作者**:Tanida, I. et al.

**摘要**:该综述系统总结了LC3蛋白家族(包括LC3B)在自噬中的分子机制,并详细分析了不同LC3B抗体的表位特异性及其适用实验类型(如免疫组化需选择识别天然构象的抗体)。

**注**:以上文献均发表于*Autophagy*等高水平期刊,可作为LC3B抗体使用及自噬研究的核心参考文献。

       

背景信息

The MAP1LC3B (microtubule-associated protein 1 light chain 3 beta) antibody is a critical tool for studying autophagy, a cellular degradation process essential for maintaining homeostasis. LC3B, a mammalian homolog of yeast Atg8. is a key marker of autophagosomes. During autophagy, cytosolic LC3B (LC3B-I) conjugates to phosphatidylethanolamine, forming LC3B-II, which integrates into autophagosomal membranes. This conversion is widely used to monitor autophagic activity.

The antibody specifically detects LC3B isoforms, distinguishing between LC3B-I (18 kDa) and LC3B-II (16 kDa) via Western blotting. It is also employed in immunofluorescence (IF) and immunohistochemistry (IHC) to visualize autophagosome formation in cells or tissues. Researchers utilize it to explore autophagy's role in diseases like cancer, neurodegenerative disorders, and infections, as dysregulated autophagy contributes to these conditions.

Commercial LC3B antibodies are typically raised against synthetic peptides corresponding to conserved regions of human LC3B, ensuring cross-reactivity with homologs in mice, rats, and other species. However, interpretation requires caution: LC3B-II levels alone may not fully represent autophagic flux, necessitating complementary assays (e.g., lysosomal inhibition). Variability in antibody specificity and batch consistency also underscores the need for validation using LC3B-knockout controls. Overall, the LC3B antibody remains indispensable for elucidating autophagy mechanisms in health and disease.

       
关键字: MAP1LC3B抗体;MAP1LC3B;MAP1LC3B Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

店内推荐

MAP1LC3B抗体相关厂家报价

产品名称 价格   公司名称 报价日期
¥1600.00
VIP10年
北京索莱宝科技有限公司
2026-01-16
¥1950
VIP4年
上海沪震实业有限公司
2026-01-16
询价
VIP4年
维百奥(北京)生物科技有限公司
2025-12-29
内容声明:
以上所展示的信息由商家自行提供,内容的真实性、准确性和合法性由发布商家负责。 商家发布价格指该商品的参考价格,并非原价,该价格可能随着市场变化,或是由于您购买数量不同或所选规格不同而发生变化。最终成交价格,请咨询商家,以实际成交价格为准。请意识到互联网交易中的风险是客观存在的
主页 | 企业会员服务 | 广告业务 | 联系我们 | 旧版入口 | 中文MSDS | CAS Index | 常用化学品CAS列表 | 化工产品目录 | 新产品列表 |投诉中心
Copyright © 2008 ChemicalBook 京ICP备07040585号  京公海网安备110108000080号  All rights reserved.